[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

[HTML][HTML] A fluoride-responsive genetic circuit enables in vivo biofluorination in engineered Pseudomonas putida

P Calero, DC Volke, PT Lowe, CH Gotfredsen… - Nature …, 2020 - nature.com
Fluorine is a key element in the synthesis of molecules broadly used in medicine, agriculture
and materials. Addition of fluorine to organic structures represents a unique strategy for …

[HTML][HTML] Growth-coupled selection of synthetic modules to accelerate cell factory development

E Orsi, NJ Claassens, PI Nikel, SN Lindner - Nature communications, 2021 - nature.com
Synthetic biology has brought about a conceptual shift in our ability to redesign microbial
metabolic networks. Combining metabolic pathway-modularization with growth-coupled …

A nonconventional archaeal fluorinase identified by in silico mining for enhanced fluorine biocatalysis

I Pardo, D Bednar, P Calero, DC Volke… - ACS …, 2022 - ACS Publications
Fluorinases, the only enzymes known to catalyze the transfer of fluorine to an organic
molecule, are essential catalysts for the biological synthesis of valuable organofluorines …

[HTML][HTML] Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation

J Turlin, B Dronsella, A De Maria, SN Lindner… - Metabolic …, 2022 - Elsevier
Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …

[HTML][HTML] Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida

E Kozaeva, S Volkova, MRA Matos, MP Mezzina… - Metabolic …, 2021 - Elsevier
Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …

[HTML][HTML] Synthetic control of plasmid replication enables target-and self-curing of vectors and expedites genome engineering of Pseudomonas putida

DC Volke, L Friis, NT Wirth, J Turlin, PI Nikel - Metabolic engineering …, 2020 - Elsevier
Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …

Pseudomonas as Versatile Aromatics Cell Factory

T Schwanemann, M Otto, N Wierckx… - Biotechnology …, 2020 - Wiley Online Library
Aromatics and their derivatives are valuable chemicals with a plethora of important
applications and thus play an integral role in modern society. Their current production relies …