Detection of genetically modified organisms in foods

FE Ahmed - TRENDS in Biotechnology, 2002 - cell.com
Legislation enacted worldwide to regulate the presence of genetically modified organisms
(GMOs) in crops, foods and ingredients, necessitated the development of reliable and …

Natural and engineered nucleic acids as tools to explore biology

RR Breaker - Nature, 2004 - nature.com
RNA and DNA molecules can form complex, three-dimensional folded structures that have
surprisingly sophisticated functions, including catalysing chemical reactions and controlling …

Universal and blocking primer mismatches limit the use of highthroughput DNA sequencing for the quantitative metabarcoding of arthropods

J Piñol, G Mir, P GomezPolo… - Molecular ecology …, 2015 - Wiley Online Library
The quantification of the biological diversity in environmental samples using high
throughput DNA sequencing is hindered by the PCR bias caused by variable primer …

Experimental validation of novel and conventional approaches to quantitative realtime PCR data analysis

SN Peirson, JN Butler, RG Foster - Nucleic acids research, 2003 - academic.oup.com
Realtime PCR is being used increasingly as the method of choice for mRNA quantification,
allowing rapid analysis of gene expression from low quantities of starting template. Despite …

Length and GC-biases during sequencing library amplification: a comparison of various polymerase-buffer systems with ancient and modern DNA sequencing …

J Dabney, M Meyer - Biotechniques, 2012 - Taylor & Francis
High-throughput sequencing technologies frequently necessitate the use of PCR for
sequencing library amplification. PCR is a sometimes enigmatic process and is known to …

[图书][B] Archaeological chemistry

Z Goffer - 2006 - books.google.com
The chemical study of archaeological materials Archaeological Chemistry, Second Edition is
about the application of the chemical sciences to the study of ancient man and his material …

Exploiting molecular methods to explore endodontic infections: part 1—current molecular technologies for microbiological diagnosis

JF Siqueira Jr, IN Rôças - Journal of endodontics, 2005 - Elsevier
Endodontic infections have been traditionally studied by culture-dependent methods.
However, as with other areas of clinical microbiology, culture-based investigations are …

A critical analysis of research methods and experimental models to study the root canal microbiome

JF Siqueira Jr, IN Rôças - International Endodontic Journal, 2022 - Wiley Online Library
Endodontic microbiology deals with the study of the microbial aetiology and pathogenesis of
pulpal and periradicular inflammatory diseases. Research in endodontic microbiology …

Diagnostic microRNA markers for screening sporadic human colon cancer and active ulcerative colitis in stool and tissue

FE Ahmed, CD Jeffries, PW Vos, G Flake… - Cancer genomics & …, 2009 - cgp.iiarjournals.org
By routinely and systematically being able to perform quantitative stem-loop reverse
transcriptase followed by TaqMan® PCR expression analysis on stool and tissue samples …

Revisiting the effect of PCR replication and sequencing depth on biodiversity metrics in environmental DNA metabarcoding

S Shirazi, RS Meyer, B Shapiro - Ecology and Evolution, 2021 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding is an increasingly popular tool for measuring
and cataloguing biodiversity. Because the environments and substrates in which DNA is …