The human transcriptome is composed of a vast RNA population that undergoes further
diversification by splicing. Detecting specific splice sites in this large sequence pool is the …

The 5′ m7G cap is an evolutionarily conserved modification of eukaryotic mRNA. Decades
of research have established that the m7G cap serves as a unique molecular module that …

Pre-mRNA maturation frequently occurs at the same time and place as transcription by RNA
polymerase II. The co-transcriptionality of mRNA processing has permitted the evolution of …

Internal membrane bound structures sequester all genetic material in eukaryotic cells. The
most prominent of these structures is the nucleus, which is bounded by a double membrane …

From sites of transcription in the nucleus to the outreaches of the cytoplasm, messenger
RNAs are associated with RNA-binding proteins. These proteins influence pre-mRNA …

SR proteins constitute a family of pre-mRNA splicing factors now thought to play several
roles in mRNA metabolism in metazoan cells. Here we provide evidence that a prototypical …

Transcription is a fundamental step in gene expression, yet it remains poorly understood at a
cellular level. Visualization of transcription sites and active genes has led to the suggestion …

In the cytosol, actin polymers, intermediate filaments and microtubules can anchor to cell
surface adhesions and interlink to form intricate networks. This cytoskeleton is anchored to …

Much of the complex process of RNP biogenesis takes place at the gene cotranscriptionally.
The target for RNA binding and processing factors is, therefore, not a solitary RNA molecule …

The eukaryotic nucleus is surrounded by a protective nuclear envelope, which is perforated
by trafficking machines termed nuclear pore complexes (NPCs). The NPCs are the sole …