A low-cost library construction protocol and data analysis pipeline for Illumina-based strand-specific multiplex RNA-seq
The emergence of NextGen sequencing technology has generated much interest in the
exploration of transcriptomes. Currently, Illumina Inc.(San Diego, CA) provides one of the …
exploration of transcriptomes. Currently, Illumina Inc.(San Diego, CA) provides one of the …
RNA-seq Profiles of Immune Related Genes in the Staghorn Coral Acropora cervicornis Infected with White Band Disease
Coral diseases are among the most serious threats to coral reefs worldwide, yet most coral
diseases remain poorly understood. How the coral host responds to pathogen infection is an …
diseases remain poorly understood. How the coral host responds to pathogen infection is an …
[HTML][HTML] Landscape of pathogenic variations in a panel of 34 genes and cancer risk estimation from 5131 HBOC families
L Castéra, V Harter, E Muller, S Krieger, N Goardon… - Genetics in …, 2018 - Elsevier
Purpose Integration of gene panels in the diagnosis of hereditary breast and ovarian cancer
(HBOC) requires a careful evaluation of the risk associated with pathogenic or likely …
(HBOC) requires a careful evaluation of the risk associated with pathogenic or likely …
Targeted next-generation sequencing for the molecular genetic diagnostics of cardiomyopathies
Background—Today, mutations in more than 30 different genes have been found to cause
inherited cardiomyopathies, some associated with very poor prognosis. However, because …
inherited cardiomyopathies, some associated with very poor prognosis. However, because …
Next-generation sequencing techniques for eukaryotic microorganisms: sequencing-based solutions to biological problems
M Nowrousian - Eukaryotic cell, 2010 - Am Soc Microbiol
Over the past 5 years, large-scale sequencing has been revolutionized by the development
of several so-called next-generation sequencing (NGS) technologies. These have drastically …
of several so-called next-generation sequencing (NGS) technologies. These have drastically …
Adapterama I: universal stubs and primers for thousands of dual-indexed Illumina libraries (iTru & iNext)
Next-generation DNA sequencing (NGS) offers many benefits, but major factors limiting NGS
include reducing the time and costs associated with: 1) start-up (ie, doing NGS for the first …
include reducing the time and costs associated with: 1) start-up (ie, doing NGS for the first …
Adapterama II: universal amplicon sequencing on Illumina platforms (TaggiMatrix)
Next-generation sequencing (NGS) of amplicons is used in a wide variety of contexts. In
many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library …
many cases, NGS amplicon sequencing remains overly expensive and inflexible, with library …
[HTML][HTML] Next generation sequencing for clinical diagnostics-principles and application to targeted resequencing for hypertrophic cardiomyopathy: a paper from the …
KV Voelkerding, S Dames, JD Durtschi - The Journal of molecular …, 2010 - Elsevier
During the past five years, new high-throughput DNA sequencing technologies have
emerged; these technologies are collectively referred to as next generation sequencing …
emerged; these technologies are collectively referred to as next generation sequencing …
DNA Sudoku—harnessing high-throughput sequencing for multiplexed specimen analysis
Next-generation sequencers have sufficient power to analyze simultaneously DNAs from
many different specimens, a practice known as multiplexing. Such schemes rely on the …
many different specimens, a practice known as multiplexing. Such schemes rely on the …
Employing genome-wide SNP discovery and genotyping strategy to extrapolate the natural allelic diversity and domestication patterns in chickpea
The genome-wide discovery and high-throughput genotyping of SNPs in chickpea natural
germplasm lines is indispensable to extrapolate their natural allelic diversity, domestication …
germplasm lines is indispensable to extrapolate their natural allelic diversity, domestication …