Intracytoplasmic injection of spermatozoa and spermatogenic cells: its biology and applications in humans and animals

R Yanagimachi - Reproductive biomedicine online, 2005 - Elsevier
Intracytoplasmic sperm injection (ICSI) has become the method of choice to overcome male
infertility when all other forms of assisted fertilization have failed. Animals in which ICSI has …

Past, present and future of ICSI in livestock species

O Briski, DF Salamone - Animal Reproduction Science, 2022 - Elsevier
During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine
technique for clinical applications in humans. The widespread use among domestic species …

Pig cloning by microinjection of fetal fibroblast nuclei

A Onishi, M Iwamoto, T Akita, S Mikawa, K Takeda… - Science, 2000 - science.org
Pig cloning will have a marked impact on the optimization of meat production and
xenotransplantation. To clone pigs from differentiated cells, we microinjected the nuclei of …

Genetic divergence in cellular resistance to heat shock in cattle: differences between breeds developed in temperate versus hot climates in responses of …

FF Paula-Lopes, CC Chase, YM Al-Katanani… - …, 2003 - rep.bioscientifica.com
The detrimental effects of heat stress on fertility in cattle are less pronounced in heat-tolerant
breeds. Although these genetic differences reflect differences in thermoregulation, cells from …

[HTML][HTML] Intracytoplasmic sperm injection in domestic and wild mammals

DF Salamone, NG Canel, MB Rodríguez - Reproduction, 2017 - rep.bioscientifica.com
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …

Activation, pronuclear formation, and development in vitro of pig oocytes following intracytoplasmic injection of freeze-dried spermatozoa

IK Kwon, KE Park, K Niwa - Biology of Reproduction, 2004 - academic.oup.com
The fertilization of pig oocytes following intracytoplasmic injection of freeze-dried
spermatozoa was evaluated. Activation and male pronuclear (MPN) formation were better in …

Production of transgenic-clone pigs by the combination of ICSI-mediated gene transfer with somatic cell nuclear transfer

M Kurome, H Ueda, R Tomii, K Naruse… - Transgenic …, 2006 - Springer
The objective of this study was to examine whether the ICSI-mediated gene transfer method
using in vitro matured oocytes and frozen sperm head could actually produce transgenic …

Incorporation of the acrosome into the oocyte during intracytoplasmic sperm injection could be potentially hazardous to embryo development

K Morozumi, R Yanagimachi - Proceedings of the National …, 2005 - National Acad Sciences
In mice and humans, a normal offspring can be obtained by injecting a single spermatozoon
into an oocyte, the process called intracytoplasmic sperm injection (ICSI). When three or …

Full-term development of golden hamster oocytes following intracytoplasmic sperm head injection

Y Yamauchi, R Yanagimachi… - Biology of …, 2002 - academic.oup.com
The golden hamster is the mammalian species in which intracytoplasmic sperm injection
(ICSI) was first tried to produce fertilized oocytes. Thus far, however, there are no reports of …

Birth of normal calves after intracytoplasmic sperm injection of bovine oocytes: a methodological approach

T Horiuchi, C Emuta, Y Yamauchi, T Oikawa… - Theriogenology, 2002 - Elsevier
Intracytoplasmic sperm injection (ICSI) is advantageous when only very few spermatozoa
are available for insemination. Bovine spermatozoa were injected individually into matured …