The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

Soybean is one of the most important oilseed and fodder crops. Benefiting from the efforts of
soybean breeders and the development of breeding technology, large number of …

Divergence of gene function is a hallmark of evolution, but assessing functional divergence
over deep time is not trivial. The few alleles available for cross-species studies often fail to …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

CRISPR-Cas has revolutionized genetic modification with its comparative simplicity and
accuracy, and it can be used even at the genomic level. Microalgae are excellent feedstocks …

Temperature is one of the decisive environmental factors that is projected to increase by 1.
5° C over the next two decades due to climate change that may affect various agronomic …

The accumulation of anthropogenic heavy metals in soil is a major form of pollution. Such
potentially toxic elements are nonbiodegradable and persist for many years as threats to …

Genome-editing tools including meganucleases, zinc finger nucleases, transcription
activator-like effector nucleases and clustered regularly interspaced short palindromic …

Multiplex genome-editing (MGE) technologies are recently developed versatile
bioengineering tools for modifying two or more specific DNA loci in a genome with high …

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-
associated (Cas) system is widely used as a genome-editing tool in various organisms …