Circular RNAs (circRNAs) are covalently closed single-stranded RNA molecules derived
from exons by alternative mRNA splicing. Circularization of single-stranded RNA molecules …

The identities of different cells and tissues in multicellular organisms are determined by
tightly controlled transcriptional programs that enable accurate gene expression. The …

Cells can be programmed to monitor and react to their environment using genetic circuits.
Design automation software maps a desired circuit function to a DNA sequence, a process …

The transcriptional machinery is thought to dissociate from DNA during replication. Certain
proteins, termed epigenetic marks, must be transferred from parent to daughter DNA strands …

The rate of transcription elongation plays an important role in the timing of expression of full-
length transcripts as well as in the regulation of alternative splicing. In this study, we coupled …

The transcription cycle can be roughly divided into three stages: initiation, elongation, and
termination. Understanding the molecular events that regulate all these stages requires a …

The synthesis of nascent RNA is a discontinuous process in which phases of productive
elongation by RNA polymerase are interrupted by frequent pauses. Transcriptional pausing …

During the humoral immune response, B cells undergo a dramatic change in phenotype to
enable antibody affinity maturation in germinal centers (GCs). Using genome-wide …

Synthetic biology may be viewed as an effort to establish, formalize, and develop an
engineering discipline in the context of biological systems. The ability to tune the properties …

Predicting how interactions between transcription factors and regulatory DNA sequence
dictate rates of transcription and, ultimately, drive developmental outcomes remains an open …