[HTML][HTML] Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

[HTML][HTML] Pseudomonas putida as a functional chassis for industrial biocatalysis: from native biochemistry to trans-metabolism

PI Nikel, V de Lorenzo - Metabolic engineering, 2018 - Elsevier
The itinerary followed by Pseudomonas putida from being a soil-dweller and plant colonizer
bacterium to become a flexible and engineer-able platform for metabolic engineering stems …

[HTML][HTML] Modular (de) construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing

DC Volke, RA Martino, E Kozaeva, AM Smania… - Nature …, 2022 - nature.com
CRISPR/Cas technologies constitute a powerful tool for genome engineering, yet their use
in non-traditional bacteria depends on host factors or exogenous recombinases, which limits …

Chasing bacterial chassis for metabolic engineering: a perspective review from classical to non‐traditional microorganisms

P Calero, PI Nikel - Microbial Biotechnology, 2019 - Wiley Online Library
The last few years have witnessed an unprecedented increase in the number of novel
bacterial species that hold potential to be used for metabolic engineering. Historically …

Reconfiguration of metabolic fluxes in Pseudomonas putida as a response to sub-lethal oxidative stress

PI Nikel, T Fuhrer, M Chavarría… - The ISME …, 2021 - academic.oup.com
As a frequent inhabitant of sites polluted with toxic chemicals, the soil bacterium and plant-
root colonizer Pseudomonas putida can tolerate high levels of endogenous and exogenous …

Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways

NL Bitzenhofer, L Kruse, S Thies… - Essays in …, 2021 - portlandpress.com
Biotechnological production in bacteria enables access to numerous valuable chemical
compounds. Nowadays, advanced molecular genetic toolsets, enzyme engineering as well …

Challenges and opportunities in biological funneling of heterogeneous and toxic substrates beyond lignin

AJ Borchert, WR Henson, GT Beckham - Current opinion in biotechnology, 2022 - Elsevier
Significant developments in the understanding and manipulation of microbial metabolism
have enabled the use of engineered biological systems toward a more sustainable energy …

[HTML][HTML] Biobased PET from lignin using an engineered cis, cis-muconate-producing Pseudomonas putida strain with superior robustness, energy and redox …

M Kohlstedt, A Weimer, F Weiland, J Stolzenberger… - Metabolic …, 2022 - Elsevier
Polyethylene terephthalate (PET), the most common synthetic polyester today, is largely
produced from fossil resources, contributing to global warming. Consequently, sustainable …

[HTML][HTML] Model-guided dynamic control of essential metabolic nodes boosts acetyl-coenzyme A–dependent bioproduction in rewired Pseudomonas putida

E Kozaeva, S Volkova, MRA Matos, MP Mezzina… - Metabolic …, 2021 - Elsevier
Pseudomonas putida is evolutionarily endowed with features relevant for bioproduction,
especially under harsh operating conditions. The rich metabolic versatility of this species …

[HTML][HTML] Predicting and improving the microbial removal of organic micropollutants during wastewater treatment: A review

AB Rios-Miguel, TJHM van Bergen, C Zillien… - Chemosphere, 2023 - Elsevier
Organic micropollutants (OMPs) consist of widely used chemicals such as pharmaceuticals
and pesticides that can persist in surface and groundwaters at low concentrations (ng/L to …