Advances in nucleic acid-based detection methods

MJ Wolcott - Clinical microbiology reviews, 1992 - Am Soc Microbiol
Laboratory techniques based on nucleic acid methods have increased in popularity over the
last decade with clinical microbiologists and other laboratory scientists who are concerned …

Analysis of genes and chromosomes by nonisotopic in situ hybridization

P Lichter, AL Boyle, T Cremer, DC Ward - Genetic Analysis: Biomolecular …, 1991 - Elsevier
Nonisotopic in situ hybridization is a powerful tool to analyze the organization of complex
genomes. Current approaches utilizing this technique for the analysis of linear and spatial …

Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase

GG Schmitz, T Walter, R Seibl, C Kessler - Analytical biochemistry, 1991 - Elsevier
A procedure for the nonradioactive labeling of oligonucleotides with the hapten digoxigenin
(DIG) has been developed. The label is introduced by enzymatic tailing of the 3′-end. Two …

[HTML][HTML] Dynamic force spectroscopy of the digoxigenin–antibody complex

G Neuert, C Albrecht, E Pamir, HE Gaub - FEBS letters, 2006 - Elsevier
Small ligands and their receptors are widely used non-covalent couplers in various biotech
applications. One prominent example, the digoxigenin–antibody complex, was often used to …

Alternative pre‐rRNA processing pathways in human cells and their alteration by cycloheximide inhibition of protein synthesis

KV Hadjiolova, M Nicoloso, S Mazan… - European journal of …, 1993 - Wiley Online Library
rRNA processing pathways in humans have been reinverstigated through systematic
Northernblot hybridizations of HeLa cell nuclear RNA with a collection of digoxigenine …

Cloning, expression, and sequencing of squalene-hopene cyclase, a key enzyme in triterpenoid metabolism

D Ochs, C Kaletta, KD Entian… - Journal of …, 1992 - Am Soc Microbiol
The pentacyclic hopanoids, a class of eubacterial lipids, are synthesized by squalene-
hopene cyclase and side chain-elongating enzymes. With the aid of DNA probes based on …

[HTML][HTML] An episomally replicating vector binds to the nuclear matrix protein SAF‐A in vivo

BHC Jenke, CP Fetzer, IM Stehle, F Jönsson… - EMBO …, 2002 - embopress.org
pEPI‐1, a vector in which a chromosomal scaffold/matrix‐attached region (S/MAR) is linked
to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the …

Bispecific digoxigenin-binding antibodies for targeted payload delivery

S Metz, AK Haas, K Daub… - Proceedings of the …, 2011 - National Acad Sciences
Bispecific antibodies that bind cell-surface targets as well as digoxigenin (Dig) were
generated for targeted payload delivery. Targeting moieties are IgGs that bind the tumor …

The digoxigenin: anti-digoxigenin (DIG) technology—a survey on the concept and realization of a novel bioanalytical indicator system

C Kessler - Molecular and cellular probes, 1991 - Elsevier
A review is given on the novel non-radioactive digoxigenin: anti-digoxigenin (DIG)
bioanalytical indicator system. After a general introduction on direct and indirect indicator …

Cloning and nucleotide sequence of the pst A gene of Wolinella succinogenes polysulphide reductase

T Krafft, M Bokranz, O Klimmek… - European journal of …, 1992 - Wiley Online Library
The polysulphide reductase (formerly sulphur reductase) of Wolinella succinogenes is a
component of the phosphorylative electron transport system with polysulphid as the terminal …