Novel CRISPR–Cas systems: an updated review of the current achievements, applications, and future research perspectives

S Nidhi, U Anand, P Oleksak, P Tripathi, JA Lal… - International journal of …, 2021 - mdpi.com
According to Darwin's theory, endless evolution leads to a revolution. One such example is
the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an …

Developing Aspergillus niger as a cell factory for food enzyme production

C Li, J Zhou, G Du, J Chen, S Takahashi, S Liu - Biotechnology Advances, 2020 - Elsevier
Aspergillus niger has become one of the most important hosts for food enzyme production
due to its unique food safety characteristics and excellent protein secretion systems. A series …

Efficient exploration of terpenoid biosynthetic gene clusters in filamentous fungi

Y Yuan, S Cheng, G Bian, P Yan, Z Ma, W Dai… - Nature Catalysis, 2022 - nature.com
Terpenoids, critical components of human medicine, are the largest family of natural
products. Fungi are an important source of terpenoids, but many of the corresponding …

CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective

R Song, Q Zhai, L Sun, E Huang, Y Zhang… - Applied microbiology …, 2019 - Springer
Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

[HTML][HTML] CRISPR-Cas9 genome editing approaches in filamentous fungi and oomycetes

M Schuster, R Kahmann - Fungal Genetics and Biology, 2019 - Elsevier
Due to their biotechnological relevance as well as their importance as disease agents,
filamentous fungi and oomycetes have been prime candidates for genetic selection and in …

CRISPR-Cas9 ribonucleoprotein-mediated co-editing and counterselection in the rice blast fungus

AJ Foster, M Martin-Urdiroz, X Yan, HS Wright… - Scientific reports, 2018 - nature.com
The rice blast fungus Magnaporthe oryzae is the most serious pathogen of cultivated rice
and a significant threat to global food security. To accelerate targeted mutation and specific …

Forced recycling of an AMA1-based genome-editing plasmid allows for efficient multiple gene deletion/integration in the industrial filamentous fungus Aspergillus …

T Katayama, H Nakamura, Y Zhang… - Applied and …, 2019 - Am Soc Microbiol
Filamentous fungi are used for food fermentation and industrial production of recombinant
proteins. They also serve as a source of secondary metabolites and are recently expected …

Advances in Genetic Engineering Technology and Its Application in the Industrial Fungus Aspergillus oryzae

FJ Jin, S Hu, BT Wang, L Jin - Frontiers in Microbiology, 2021 - frontiersin.org
The filamentous fungus Aspergillus oryzae is an important strain in the traditional
fermentation and food processing industries and is often used in the production of soy …

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger

L Song, JP Ouedraogo, M Kolbusz, TTM Nguyen… - PloS one, 2018 - journals.plos.org
As a powerful tool for fast and precise genome editing, the CRISPR/Cas9 system has been
applied in filamentous fungi to improve the efficiency of genome alteration. However, the …

[HTML][HTML] Tailored biosynthesis of gibberellin plant hormones in yeast

KR Kildegaard, JA Arnesen, B Adiego-Pérez… - Metabolic …, 2021 - Elsevier
The application of small amounts of natural plant growth hormones, such as gibberellins
(GAs), can increase the productivity and quality of many vegetable and fruit crops. However …