Molecular diagnostics for human leptospirosis
JJ Waggoner, BA Pinsky - Current opinion in infectious diseases, 2016 - journals.lww.com
Molecular diagnostics have an established role for the diagnosis of leptospirosis and
provide an actionable diagnosis in the acute setting. The use of real-time reverse …
provide an actionable diagnosis in the acute setting. The use of real-time reverse …
Evaluation of diagnostic accuracy of loop-mediated isothermal amplification method (LAMP) compared with polymerase chain reaction (PCR) for Leptospira spp. in …
S Gunasegar, VK Neela - Diagnostic microbiology and infectious disease, 2021 - Elsevier
Loop-mediated isothermal amplification (LAMP) test is widely used in molecular diagnostics
as a point-of-care technique alternative to traditional PCR especially in resource-limited …
as a point-of-care technique alternative to traditional PCR especially in resource-limited …
Diagnosis of Human Leptospirosis in a Clinical Setting: Real-Time PCR High Resolution Melting Analysis for Detection of Leptospira at the Onset of Disease
LM Esteves, SM Bulhões, CC Branco, T Carreira… - Scientific reports, 2018 - nature.com
Currently, direct detection of Leptospira can be done in clinical laboratories by conventional
and by real-time PCR (qRT-PCR). We tested a biobank of paired samples of serum and …
and by real-time PCR (qRT-PCR). We tested a biobank of paired samples of serum and …
Laboratory diagnostic, epidemiological, and clinical characteristics of human leptospirosis in Okinawa Prefecture, Japan, 2003–2020
T Kakita, S Okano, H Kyan, M Miyahira… - PLoS Neglected …, 2021 - journals.plos.org
Background Leptospirosis is considered an endemic disease among agricultural workers in
Okinawa Prefecture, which is the southernmost part of Japan and has a subtropical climate …
Okinawa Prefecture, which is the southernmost part of Japan and has a subtropical climate …
[HTML][HTML] Dynamics of spirochetemia and early PCR detection of Borrelia miyamotoi
L Karan, M Makenov, N Kolyasnikova… - Emerging infectious …, 2018 - ncbi.nlm.nih.gov
We investigated whether Borrelia miyamotoi disease can be detected in its early stage by
using PCR for borrelial 16S rRNA, which molecule (DNA or RNA) is the best choice for this …
using PCR for borrelial 16S rRNA, which molecule (DNA or RNA) is the best choice for this …
Leptospira enrichment culture followed by ONT metagenomic sequencing allows better detection of Leptospira presence and diversity in water and soil samples
M Gorman, R Xu, D Prakoso… - PLOS Neglected …, 2022 - journals.plos.org
Background Leptospirosis, a life-threatening disease in humans and animals, is one of the
most widespread global zoonosis. Contaminated soil and water are the major transmission …
most widespread global zoonosis. Contaminated soil and water are the major transmission …
Leptospira diversity in animals and humans in Tahiti, French Polynesia
V Guernier, V Richard, T Nhan, E Rouault… - PLoS neglected …, 2017 - journals.plos.org
Background Leptospirosis is a highly endemic bacterial zoonosis in French Polynesia (FP).
Nevertheless, data on the epidemiology of leptospirosis in FP are scarce. We conducted …
Nevertheless, data on the epidemiology of leptospirosis in FP are scarce. We conducted …
Ultrasensitive Detection of Bacillus anthracis by Real-Time PCR Targeting a Polymorphism in Multi-Copy 16S rRNA Genes and Their Transcripts
The anthrax pathogen Bacillus anthracis poses a significant threat to human health.
Identification of B. anthracis is challenging because of the bacterium's close genetic …
Identification of B. anthracis is challenging because of the bacterium's close genetic …
Isolation and clinical sample typing of human leptospirosis cases in Argentina
Y Chiani, P Jacob, V Varni, N Landolt… - Infection, Genetics and …, 2016 - Elsevier
Leptospira typing is carried out using isolated strains. Because of difficulties in obtaining
them, direct identification of infective Leptospira in clinical samples is a high priority …
them, direct identification of infective Leptospira in clinical samples is a high priority …
Laboratory diagnosis of leptospirosis
N Koizumi - Leptospira spp. Methods and Protocols, 2020 - Springer
The diagnosis of leptospirosis depends on specific laboratory tests because nonspecific and
diverse clinical manifestations make clinical diagnosis difficult and it is easily confused with …
diverse clinical manifestations make clinical diagnosis difficult and it is easily confused with …