CRISPR in livestock: From editing to printing

A Menchaca, PC Dos Santos-Neto, AP Mulet, M Crispo - Theriogenology, 2020 - Elsevier
Precise genome editing of large animals applied to livestock and biomedicine is nowadays
possible since the CRISPR revolution. This review summarizes the latest advances and the …

Towards improving the outcomes of assisted reproductive technologies of cattle and sheep, with particular focus on recipient management

J Daly, H Smith, HA McGrice, KL Kind… - Animals, 2020 - mdpi.com
Simple Summary The Australian livestock industry has grown significantly over the last
decade. In order to meet national consumer demands, as well as the growing export market …

[HTML][HTML] From reproductive technologies to genome editing in small ruminants: An embryo's journey

A Menchaca, PC dos Santos-Neto, F Cuadro… - Animal …, 2018 - ncbi.nlm.nih.gov
The beginning of this century has witnessed great advances in the understanding of ovarian
physiology and embryo development, in the improvement of assisted reproductive …

Generation of double-muscled sheep and goats by CRISPR/Cas9-mediated knockout of the myostatin gene

P Kalds, M Crispo, C Li, L Tesson, I Anegon… - Applications of Genome …, 2022 - Springer
The myostatin (MSTN) gene has shown to play a critical role in the regulation of skeletal
muscle mass, and the translational inhibition of this gene has shown increased muscle …

Embryo survival and birth rate after minimum volume vitrification or slow freezing of in vivo and in vitro produced ovine embryos

PC Dos Santos-Neto, F Cuadro, N Barrera, M Crispo… - Cryobiology, 2017 - Elsevier
The objective was to evaluate pregnancy outcomes and birth rate of in vivo derived vs. in
vitro produced ovine embryos submitted to different cryopreservation methods. A total of 197 …

Redesigning small ruminant genomes with CRISPR toolkit: overview and perspectives

P Kalds, Y Gao, S Zhou, B Cai, X Huang, X Wang… - Theriogenology, 2020 - Elsevier
Genetic modification is a rapidly developing field in which numerous significant
breakthroughs have been achieved. Over the last few decades, genetic modification has …

Impact of delipidated estrous sheep serum supplementation on in vitro maturation, cryotolerance and endoplasmic reticulum stress gene expression of sheep oocytes

N Barrera, PC dos Santos Neto, F Cuadro… - PLoS …, 2018 - journals.plos.org
High lipid content of oocytes and embryos in domestic animals is one of the well-known
factors associated with poor cryosurvival. Herein, we wanted to determine whether the use …

Oocyte developmental competence is improved by relatively greater circulating progesterone concentrations during preovulatory follicular growth

A Menchaca, F Cuadro, PC dos Santos-Neto… - Animal reproduction …, 2018 - Elsevier
This study evaluated the effect of progesterone priming during follicular growth on oocyte
competence to undergo oocyte cleavage and embryo development in sheep. Two …

In vitro production of small ruminant embryos: Latest improvements and further research

JMG Souza-Fabjan, RITP Batista… - Reproduction …, 2021 - CSIRO Publishing
This review presents the latest advances in and main obstacles to the application of in vitro
embryo production (IVEP) systems in small ruminants. This biotechnology is an extremely …

Impact of short nutrient stimuli with different energy source on follicle dynamics and quality of oocyte from hormonally stimulated goats

JPM Alves, CCL Fernandes, R Rossetto… - … in Domestic Animals, 2019 - Wiley Online Library
The aim of this study was to verify the effect of the energy source for a short‐term diet
supplementation on follicular dynamics, ovarian response and oocyte recovery in goats …