CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Genetically engineered plants have varied applications in agriculture for enhancing the
values of food and feed. Genetic engineering aims to introduce selected genetic regions …

The recent discovery of the mode of action of the CRISPR/Cas9 system has provided
biologists with a useful tool for generating site-specific mutations in genes of interest. In …

Sweet orange (Citrus sinensis) is the most economically important species for the citrus
industry. However, it is susceptible to many diseases including citrus bacterial canker …

Genome editing by RNA‐guided nucleases, such as SpCas9, has been used in numerous
different plant species. However, to what extent multiple independent loci can be targeted …

Background Precision genome mutagenesis using CRISPR/Cas has become the standard
method to generate mutant plant lines. Several improvements have been made to increase …

The CRISPR/Cas9-mediated genome editing system has been used extensively to engineer
targeted mutations in a wide variety of species. Its application in banana, however, has been …

Tandemly arrayed genes (TAGs) with functional redundancy and chromosomal linkage
constitute 14~ 35% in sequenced plant genomes. The multiplex CRISPR system is the tool …

Many of the world's most important crops are polyploid. The presence of more than two sets
of chromosomes within their nuclei and frequently aberrant reproductive biology in …

Due to the economic and ecological importance of forest trees, modern breeding and
genetic manipulation of forest trees have become increasingly prevalent. The CRISPR …