Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets.
Such" onco-lncRNAs" have resisted traditional RNAi methods, but CRISPR-Cas9 genome …

A major challenge in genetics is to identify genetic variants driving natural phenotypic
variation. However, current methods of genetic mapping have limited resolution. To address …

In the first wave of synthetic biology, genetic elements, combined into simple circuits, are
used to control individual cellular functions. In the second wave of synthetic biology, the …

Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …

A fundamental challenge in biology is understanding the molecular details of protein
function. How mutations alter protein activity, regulation, and response to drugs is of critical …

Clustered regularly interspaced short palindromic repeat (CRISPR)-associated systems
have revolutionized genome engineering by facilitating a wide range of targeted DNA …

Metabolic engineering holds the promise to transform the chemical industry and to support
the transition into a circular bioeconomy, by engineering cellular biocatalysts that efficiently …

High-throughput genetic screens interfering with gene expression are invaluable tools to
identify gene function and phenotype-to-genotype interactions. Implementing such screens …

Abstract Wheat (Triticum aestivum, 2n= 6x= 42, AABBDD) is one of the most important staple
food crops in the world. Despite the fact that wheat production has significantly increased …