CRISPR/Cas, as a simple, versatile, robust and cost-effective system for genome
manipulation, has dominated the genome editing field over the past few years. The …

Post‐translational modifications (PTMs) are critical regulators of protein function, and nearly
200 different types of PTM have been identified. Advances in high‐resolution mass …

The phenotypic effect of any genetic variant can be altered by variation at other genomic
loci. Known as epistasis, these genetic interactions shape the genotype-phenotype map of …

Highlights•Directed genome evolution is a powerful tool to unlock microbial biosynthetic
potential.•Adaptive laboratory evolution is a traditional but efficient tool to improve cell …

DNA variants that alter gene expression contribute to variation in many phenotypic traits. In
particular, trans-acting variants, which are often located on different chromosomes from the …

INTRODUCTION Determining the structures of protein complexes is crucial for
understanding cellular functions. Here, we describe an integrative structure determination …

With the rapid development of DNA synthesis and next-generation sequencing, synthetic
biology that aims to standardize, modularize, and innovate cellular functions, has achieved …

Most traits of human interest vary among individuals within a population due to a
combination of genetic and environmental factors, meaning individuals' phenotypes will …

Genomic variants libraries are conducive to obtain dominant strains with desirable
phenotypic traits. The non-homologous end joining (NHEJ), which enables foreign DNA …

The CRISPR/Cas9 systems have been developed as tools for genetic engineering and
metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 …