Pathogenic fungi represent an increasing infectious disease threat to humans, especially
with an increasing challenge of antifungal drug resistance. Over the decades, numerous …

Until recently, our ability to generate allelic diversity in plants was limited to introduction of
variants from domesticated and wild species by breeding via uncontrolled recombination or …

The hydrophobicity of lipids prevents their free movement across the cytoplasm. To achieve
highly heterogeneous and precisely regulated lipid distribution in different cellular …

Undergraduate students participating in the UCLA Undergraduate Research Consortium for
Functional Genomics (URCFG) have conducted a two-phased screen using RNA …

Spontaneous and induced mutations frequently show different phenotypic effects across
genetically distinct individuals. It is generally appreciated that these background effects …

Retron library recombineering (RLR) is a powerful tool in the field of genome editing that
exceeds the scope and specificity of the CRISPR/Cas technique. In RLR, single-stranded …

The occurrence of random mutations can increase the diversity of the genome and promote
the evolutionary process of organisms. High efficiency mutagenesis techniques significantly …

Genome mutagenesis drives the evolution of organisms. Here, we developed a C RISPR-
Cas a ssisted r andom m utation (CARM) technique for whole-genome mutagenesis. The …

As the most thoroughly investigated eukaryotic microorganism, Saccharomyces cerevisiae is
widely used as a cell factory for producing valuable compounds. Currently, strain …

The emergence of fungal “superbugs” resistant to the limited cohort of anti-fungal agents
available to clinicians is eroding our ability to effectively treat infections by these virulent …