Machine learning methods for fluorescence lifetime imaging (FLIM) based label-free detection of microglia
MAK Sagar, KP Cheng, JN Ouellette… - Frontiers in …, 2020 - frontiersin.org
Automated computational analysis techniques utilizing machine learning have been
demonstrated to be able to extract more data from different imaging modalities compared to …
demonstrated to be able to extract more data from different imaging modalities compared to …
A shift in central metabolism accompanies virulence activation in Pseudomonas aeruginosa
The availability of energy has significant impact on cell physiology. However, the role of
cellular metabolism in bacterial pathogenesis is not understood. We investigated the …
cellular metabolism in bacterial pathogenesis is not understood. We investigated the …
Applying phasor approach analysis of multiphoton FLIM measurements to probe the metabolic activity of three-dimensional in vitro cell culture models
PH Lakner, MG Monaghan, Y Möller, MA Olayioye… - Scientific Reports, 2017 - nature.com
Fluorescence lifetime imaging microscopy (FLIM) can measure and discriminate
endogenous fluorophores present in biological samples. This study seeks to identify FLIM as …
endogenous fluorophores present in biological samples. This study seeks to identify FLIM as …
Metabolic imaging using two-photon excited NADH intensity and fluorescence lifetime imaging
J Vergen, C Hecht, LV Zholudeva… - Microscopy and …, 2012 - academic.oup.com
Metabolism and mitochondrial dysfunction are known to be involved in many different
disease states. We have employed two-photon fluorescence imaging of intrinsic …
disease states. We have employed two-photon fluorescence imaging of intrinsic …
Autofluorescence imaging of 3D tumor–macrophage microscale cultures resolves spatial and temporal dynamics of macrophage metabolism
Macrophages within the tumor microenvironment (TME) exhibit a spectrum of protumor and
antitumor functions, yet it is unclear how the TME regulates this macrophage heterogeneity …
antitumor functions, yet it is unclear how the TME regulates this macrophage heterogeneity …
NAD (P) H autofluorescence lifetime imaging enables single cell analyses of cellular metabolism of osteoblasts in vitro and in vivo via two-photon microscopy
K Schilling, E Brown, X Zhang - Bone, 2022 - Elsevier
Two-photon fluorescence lifetime microscopy (2P-FLIM) is a non-invasive optical technique
that can obtain cellular metabolism information based on the intrinsic autofluorescence …
that can obtain cellular metabolism information based on the intrinsic autofluorescence …
Multicomponent analysis of phasor plot in a single pixel to calculate changes of metabolic trajectory in biological systems
Phasor FLIM in cells undergoing oxidative stress and in mice liver sections have shown the
presence of a third autofluorescent component indicative of lipid droplets along with free and …
presence of a third autofluorescent component indicative of lipid droplets along with free and …
Metabolic state of human blastocysts measured by fluorescence lifetime imaging microscopy
STUDY QUESTION Can non-invasive metabolic imaging via fluorescence lifetime imaging
microscopy (FLIM) detect variations in metabolic profiles between discarded human …
microscopy (FLIM) detect variations in metabolic profiles between discarded human …
Drosophila TRIM32 cooperates with glycolytic enzymes to promote cell growth
Cell growth and/or proliferation may require the reprogramming of metabolic pathways,
whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates …
whereby a switch from oxidative to glycolytic metabolism diverts glycolytic intermediates …
Metabolic imaging of head and neck cancer organoids
AT Shah, TM Heaster, MC Skala - PloS one, 2017 - journals.plos.org
Head and neck cancer patients suffer from toxicities, morbidities, and mortalities, and these
ailments could be minimized through improved therapies. Drug discovery is a long …
ailments could be minimized through improved therapies. Drug discovery is a long …