Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Recent development of carbon quantum dots regarding their optical properties, photoluminescence mechanism, and core structure

KJ Mintz, Y Zhou, RM Leblanc - Nanoscale, 2019 - pubs.rsc.org
Carbon quantum dots (CDs) are a relatively new class of carbon nanomaterials which have
been studied very much in the last fifteen years to improve their already favorable properties …

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

NR Mohamad, NHC Marzuki, NA Buang… - Biotechnology & …, 2015 - Taylor & Francis
The current demands of sustainable green methodologies have increased the use of
enzymatic technology in industrial processes. Employment of enzyme as biocatalysts offers …

Chemistry, biology, and medicine of fluorescent nanomaterials and related systems: new insights into biosensing, bioimaging, genomics, diagnostics, and therapy

J Yao, M Yang, Y Duan - Chemical reviews, 2014 - ACS Publications
2. INTRODUCTION The investigation of many fundamental processes in life sciences
requires straightforward tools for fast, sensitive, reliable, and reproducible detection of …

Concurrent activation of striatal direct and indirect pathways during action initiation

G Cui, SB Jun, X Jin, MD Pham, SS Vogel… - Nature, 2013 - nature.com
The basal ganglia are subcortical nuclei that control voluntary actions, and they are affected
by a number of debilitating neurological disorders,,,. The prevailing model of basal ganglia …

[图书][B] Principles of fluorescence spectroscopy

JR Lakowicz - 2006 - Springer
The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

Advanced time-correlated single photon counting techniques for spectroscopy and imaging in biomedical systems

W Becker, A Bergmann, GL Biscotti… - … Applications of Ultrafast …, 2004 - spiedigitallibrary.org
Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

[HTML][HTML] Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

RB Sekar, A Periasamy - The Journal of cell biology, 2003 - ncbi.nlm.nih.gov
The current advances in fluorescence microscopy, coupled with the development of new
fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful …

Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application

M Stanisavljevic, S Krizkova, M Vaculovicova… - Biosensors and …, 2015 - Elsevier
Fluorescence resonance energy transfer (FRET) in combination with quantum dots (QDs)
and their superior properties has enabled designing of the new and improved sensors. In …

Multi-target spectrally resolved fluorescence lifetime imaging microscopy

T Niehörster, A Löschberger, I Gregor, B Krämer… - Nature …, 2016 - nature.com
We introduce a pattern-matching technique for efficient identification of fluorophore ratios in
complex multidimensional fluorescence signals using reference fluorescence decay and …