Precise plant genome editing using base editors and prime editors

KA Molla, S Sretenovic, KC Bansal, Y Qi - Nature Plants, 2021 - nature.com
The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

Application of CRISPR/Cas9 in crop quality improvement

Q Liu, F Yang, J Zhang, H Liu, S Rahman… - International Journal of …, 2021 - mdpi.com
The various crop species are major agricultural products and play an indispensable role in
sustaining human life. Over a long period, breeders strove to increase crop yield and …

Base editing in crops: current advances, limitations and future implications

R Mishra, RK Joshi, K Zhao - Plant Biotechnology Journal, 2020 - Wiley Online Library
Targeted mutagenesis via genome‐editing technologies holds great promise in developing
improved crop varieties to meet future demands. Point mutations or single nucleotide …

CRISPR-based genome editing for nutrient enrichment in crops: a promising approach toward global food security

D Kumar, A Yadav, R Ahmad, UN Dwivedi… - Frontiers in …, 2022 - frontiersin.org
The global malnutrition burden imparts long-term developmental, economic, social, and
medical consequences to individuals, communities, and countries. The current …

Genome editing for plant research and crop improvement

X Zhan, Y Lu, JK Zhu, JR Botella - Journal of Integrative Plant …, 2021 - Wiley Online Library
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

Advances in crop breeding through precision genome editing

G Nerkar, S Devarumath, M Purankar, A Kumar… - Frontiers in …, 2022 - frontiersin.org
The global climate change and unfavourable abiotic and biotic factors are limiting
agricultural productivity and therefore intensifying the challenges for crop scientists to meet …

[HTML][HTML] CRISPR/Cas9-mediated genome editing techniques and new breeding strategies in cereals–current status, improvements, and perspectives

S Ahmar, G Hensel, D Gruszka - Biotechnology Advances, 2023 - Elsevier
Cereal crops, including triticeae species (barley, wheat, rye), as well as edible cereals
(wheat, corn, rice, oat, rye, sorghum), are significant suppliers for human consumption …

Engineering plants using diverse CRISPR-associated proteins and deregulation of genome-edited crops

QU Zaman, A Raza, J Lozano-Juste, L Chao… - Trends in …, 2023 - cell.com
The CRISPR/Cas system comprises RNA-guided nucleases, the target specificity of which is
directed by Watson–Crick base pairing of target loci with single guide (sg) RNA to induce the …

[HTML][HTML] A CRISPR‐Cas9‐mediated domain‐specific base‐editing screen enables functional assessment of ACCase variants in rice

X Liu, R Qin, J Li, S Liao, T Shan, R Xu… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
accepted 15 January 2020.* Correspondence (Tel 86-551-65160535-807; fax 86-551-
65160535-805; email Weipengcheng@ gmail. com)(PW) and (Tel 86-551-65787986; fax 86 …

PnB Designer: a web application to design prime and base editor guide RNAs for animals and plants

SM Siegner, ME Karasu, MS Schröder, Z Kontarakis… - BMC …, 2021 - Springer
Background The rapid expansion of the CRISPR toolbox through tagging effector domains
to either enzymatically inactive Cas9 (dCas9) or Cas9 nickase (nCas9) has led to several …