Reverse transcription and integration are the defining features of the Retroviridae; the
common name “retrovirus” derives from the fact that these viruses use a virally encoded …

A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy
of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the …

Candidate enhancers can be identified on the basis of chromatin modifications, the binding
of chromatin modifiers and transcription factors and cofactors, or chromatin accessibility …

A retroviral vector system based on the human immunodeficiency virus (HIV) was developed
that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous …

We describe the construction of a safe, replication-defective and efficient lentiviral vector
suitable for in vivo gene delivery. The reverse transcription of the vector was found to be a …

More than two decades have passed since genetically modified HIV was used for gene
delivery. Through continuous improvements these early marker gene-carrying HIVs have …

Integration is vital to retroviral replication and influences the establishment of the latent HIV
reservoir. HIV-1 integration favors active genes, which is in part determined by the …

Achieving the full potential of zinc-finger nucleases (ZFNs) for genome engineering in
human cells requires their efficient delivery to the relevant cell types. Here we exploited the …

The integration of a DNA copy of the viral RNA genome into host chromatin is the defining
step of retroviral replication. This enzymatic process is catalyzed by the virus-encoded …

Cellular senescence prevents the proliferation of cells at risk for neoplastic transformation.
Nonetheless, the senescence response is thought to be antagonistically pleiotropic and thus …