HIV-1 reverse transcription
WS Hu, SH Hughes - Cold Spring Harbor …, 2012 - perspectivesinmedicine.cshlp.org
Reverse transcription and integration are the defining features of the Retroviridae; the
common name “retrovirus” derives from the fact that these viruses use a virally encoded …
common name “retrovirus” derives from the fact that these viruses use a virally encoded …
Structure and function of retroviral integrase
GN Maertens, AN Engelman… - Nature Reviews …, 2022 - nature.com
A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy
of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the …
of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the …
A systematic comparison reveals substantial differences in chromosomal versus episomal encoding of enhancer activity
Candidate enhancers can be identified on the basis of chromatin modifications, the binding
of chromatin modifiers and transcription factors and cofactors, or chromatin accessibility …
of chromatin modifiers and transcription factors and cofactors, or chromatin accessibility …
In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector
A retroviral vector system based on the human immunodeficiency virus (HIV) was developed
that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous …
that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous …
Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector.
We describe the construction of a safe, replication-defective and efficient lentiviral vector
suitable for in vivo gene delivery. The reverse transcription of the vector was found to be a …
suitable for in vivo gene delivery. The reverse transcription of the vector was found to be a …
Lentiviral vectors: basic to translational
More than two decades have passed since genetically modified HIV was used for gene
delivery. Through continuous improvements these early marker gene-carrying HIVs have …
delivery. Through continuous improvements these early marker gene-carrying HIVs have …
A critical role for alternative polyadenylation factor CPSF6 in targeting HIV-1 integration to transcriptionally active chromatin
GA Sowd, E Serrao, H Wang, W Wang… - Proceedings of the …, 2016 - National Acad Sciences
Integration is vital to retroviral replication and influences the establishment of the latent HIV
reservoir. HIV-1 integration favors active genes, which is in part determined by the …
reservoir. HIV-1 integration favors active genes, which is in part determined by the …
Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery
Achieving the full potential of zinc-finger nucleases (ZFNs) for genome engineering in
human cells requires their efficient delivery to the relevant cell types. Here we exploited the …
human cells requires their efficient delivery to the relevant cell types. Here we exploited the …
Retroviral DNA integration
P Lesbats, AN Engelman, P Cherepanov - Chemical reviews, 2016 - ACS Publications
The integration of a DNA copy of the viral RNA genome into host chromatin is the defining
step of retroviral replication. This enzymatic process is catalyzed by the virus-encoded …
step of retroviral replication. This enzymatic process is catalyzed by the virus-encoded …
Secretion of vascular endothelial growth factor by primary human fibroblasts at senescence
JP Coppé, K Kauser, J Campisi… - Journal of Biological …, 2006 - ASBMB
Cellular senescence prevents the proliferation of cells at risk for neoplastic transformation.
Nonetheless, the senescence response is thought to be antagonistically pleiotropic and thus …
Nonetheless, the senescence response is thought to be antagonistically pleiotropic and thus …