Studying within-species variation has traditionally been limited to culturable bacterial
isolates and low-resolution microbial community fingerprinting. Metagenomic sequencing …

Pathogen whole-genome sequencing has become an important tool for understanding the
transmission and epidemiology of infectious diseases. It has improved our understanding of …

Despite enhanced infection prevention efforts, Clostridioides difficile remains the leading
cause of healthcare-associated infections in the United States. Current prevention strategies …

Objective Previously published guidelines provided comprehensive recommendations for
detecting and preventing healthcare-associated infections (HAIs). The intent of this …

Healthcare units consist of numerous people circulating daily, such as workers, patients, and
companions, and these people are vehicles for the transmission of microorganisms, such as …

Background Limited information is available on the natural history of Clostridioides difficile
colonization and infection in patients with new acquisition of C. difficile in healthcare …

Clostridioides difficile is an important nosocomial pathogen increasingly observed in the
community and in different non-human reservoirs. The epidemiology and transmissibility of …

Gut microbiota composition in patients with Clostridioides difficile colonization is not well
investigated. We aimed to identify bacterial signatures associated with resistance and …

Importance Health care–associated infections are often caused by multidrug-resistant
organisms and substantially factor into hospital costs and avoidable iatrogenic harm …

To trace the routes and frequencies of transmission of Clostridioides difficile in a tertiary-care
hospital in Madrid (Spain), we sequenced the genomes from all C. difficile isolates collected …