Pig embryos can be cultured using a number of different strategies including complex
approaches like culture in vivo in a surrogate oviduct (rabbit, sheep, mouse), culture in …

BACKGROUND The advances in the world of IVF during the last decades have been rapid
and impressive and culture media play a major role in this success. Until the 1980s fertility …

Transcriptional control requires epigenetic changes directed by mitochondrial tricarboxylic
acid (TCA) cycle metabolites. In the mouse embryo, global epigenetic changes occur during …

Simplex optimization has generated several media that have improved the development of
mouse preimplantation embryos in vitro. One objective of this study was to compare the …

Considerable advances in media development for the culture of preimplantation mammalian
embryos have been made since mouse embryos were first cultured and successfully …

We have recently reported the use of sequential simplex optimization methods to design a
medium (SOM) that overcomes the block to development beyond two cells which occurs in …

In vitro development of early porcine embryos under different culture conditions was
evaluated and compared to in vivo development. First, one-and two-cell embryos were …

BACKGROUND: Failed fertilization occurs in 2–3% of ICSI cycles and is mainly due to lack
of oocyte activation. Heterologous ICSI of patient's sperm in mouse oocytes allows …

There are many examples in assisted reproduction technology, where new technology and
methods have been introduced into the clinical setting without appropriate development and …

The success of clinical IVF was initially compromised by sub-optimal culture conditions,
resulting in impaired embryo development (1–6) and a subsequent loss of viability …