Recent experimental developments in genome-wide RNA quantification hold considerable
promise for systems biology. However, rigorously probing the biology of living cells requires …

Pancreatic ductal adenocarcinoma (PDAC) is the most common type of pancreatic cancer,
characterized by high tumor heterogeneity and a poor prognosis. Inter-and intra-tumoral …

Motivation Gene expression is characterized by stochastic bursts of transcription that occur
at brief and random periods of promoter activity. The kinetics of gene expression burstiness …

Summary The Chemical Master Equation (CME) provides an accurate description of
stochastic biochemical reaction networks in well-mixed conditions, but it cannot be solved …

The snapshot distribution of mRNA counts per cell can be measured using single-molecule
fluorescence in situ hybridization or single-cell RNA sequencing. These distributions are …

Gene expression has inherent stochasticity resulting from transcription's burst manners.
Single-cell snapshot data can be exploited to rigorously infer transcriptional burst kinetics …

Single-molecule imaging inside living cells has revealed that transcription factors (TFs) bind
to DNA transiently, but a long-standing question is how this transient binding is related to …

The serial nature of reactions involved in the RNA life-cycle motivates the incorporation of
delays in models of transcriptional dynamics. The models couple a transcriptional process to …

Fluctuations in the number of nascent RNA accurately reflect transcriptional activity.
However, mathematical models predicting their distributions are difficult to solve analytically …

The mRNA life cycle is a complex biochemical process, involving transcription initiation,
elongation, termination, splicing, and degradation. Each of these molecular events is …