Extracellular vesicle analysis

A Hendrix, L Lippens, C Pinheiro, C Théry… - Nature Reviews …, 2023 - nature.com
Cells release small, phospholipid membrane-enclosed particles, collectively referred to as
extracellular vesicles (EVs), into their surroundings to enable intercellular communication …

The ins-and-outs of exosome biogenesis, secretion, and internalization

SB Arya, SP Collie, CA Parent - Trends in Cell Biology, 2023 - cell.com
Exosomes are specialized cargo delivery vesicles secreted from cells by fusion of
multivesicular bodies (MVBs) with the plasma membrane (PM). While the function of …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

Therapeutic potential in rheumatic diseases of extracellular vesicles derived from mesenchymal stromal cells

GM Bertolino, M Maumus, C Jorgensen… - Nature Reviews …, 2023 - nature.com
The incidence of rheumatic diseases such as rheumatoid arthritis and osteoarthritis and
injuries to articular cartilage that lead to osteochondral defects is predicted to rise as a result …

Cholesterol removal improves performance of a model biomimetic system to co-deliver a photothermal agent and a STING agonist for cancer immunotherapy

L Li, M Zhang, J Li, T Liu, Q Bao, X Li, J Long… - Nature …, 2023 - nature.com
Biological membranes often play important functional roles in biomimetic drug delivery
systems. We discover that the circulation time and targeting capability of biological …

[HTML][HTML] Glycosylation in extracellular vesicles: Isolation, characterization, composition, analysis and clinical applications

V Vrablova, N Kosutova, A Blsakova, A Bertokova… - Biotechnology …, 2023 - Elsevier
This review provides a comprehensive overview of our understanding of the role that
glycans play in the formation, loading and release of extracellular vesicles (EVs). The …

Large‐scale heparin‐based bind‐and‐elute chromatography identifies two biologically distinct populations of extracellular vesicles

Y Zhou, R Yuan, AS Cone, KW Shifflett… - Journal of …, 2023 - Wiley Online Library
Purifying extracellular vesicles (EVs) has been challenging because EVs are
heterogeneous in cargo yet share similar sizes and densities. Most surface marker‐based …

Exploring the versatility of exosomes: a review on isolation, characterization, detection methods, and diverse applications

O Altıntaş, Y Saylan - Analytical Chemistry, 2023 - ACS Publications
Extracellular vesicles (EVs) are crucial mediators of intercellular communication and can be
classified based on their physical properties, biomolecular structure, and origin. Among EVs …

A perspective on cross‐kingdom RNA interference in mutualistic symbioses

SA Qiao, Z Gao, R Roth - New Phytologist, 2023 - Wiley Online Library
RNA interference (RNAi) is arguably one of the more versatile mechanisms in cell biology,
facilitating the fine regulation of gene expression and protection against mobile genomic …

Biophysical and proteomic analyses of Pseudomonas syringae pv. tomato DC3000 extracellular vesicles suggest adaptive functions during plant infection

M Janda, K Rybak, L Krassini, C Meng… - MBio, 2023 - Am Soc Microbiol
Vesiculation is a process employed by Gram-negative bacteria to release extracellular
vesicles (EVs) into the environment. EVs from pathogenic bacteria play functions in host …