Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances
Engineered human cardiac tissues facilitate progress in regenerative medicine, disease
modelling and drug development. In this Perspective, we reflect on the most notable …
modelling and drug development. In this Perspective, we reflect on the most notable …
[HTML][HTML] Mitochondria and metabolic transitions in cardiomyocytes: lessons from development for stem cell-derived cardiomyocytes
JC Garbern, RT Lee - Stem Cell Research & Therapy, 2021 - Springer
Current methods to differentiate cardiomyocytes from human pluripotent stem cells (PSCs)
inadequately recapitulate complete development and result in PSC-derived cardiomyocytes …
inadequately recapitulate complete development and result in PSC-derived cardiomyocytes …
Metabolically driven maturation of human-induced-pluripotent-stem-cell-derived cardiac microtissues on microfluidic chips
The immature physiology of cardiomyocytes derived from human induced pluripotent stem
cells (hiPSCs) limits their utility for drug screening and disease modelling. Here we show …
cells (hiPSCs) limits their utility for drug screening and disease modelling. Here we show …
[HTML][HTML] Multi-chamber cardioids unravel human heart development and cardiac defects
C Schmidt, A Deyett, T Ilmer, S Haendeler… - Cell, 2023 - cell.com
The number one cause of human fetal death are defects in heart development. Because the
human embryonic heart is inaccessible and the impacts of mutations, drugs, and …
human embryonic heart is inaccessible and the impacts of mutations, drugs, and …
[HTML][HTML] Modeling human multi-lineage heart field development with pluripotent stem cells
The cardiomyocyte (CM) subtypes in the mammalian heart derive from distinct lineages
known as the first heart field (FHF), the anterior second heart field (aSHF), and the posterior …
known as the first heart field (FHF), the anterior second heart field (aSHF), and the posterior …
[HTML][HTML] PPARdelta activation induces metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes
NM Wickramasinghe, D Sachs, B Shewale… - Cell Stem Cell, 2022 - cell.com
Pluripotent stem-cell-derived cardiomyocytes (PSC-CMs) provide an unprecedented
opportunity to study human heart development and disease, but they are functionally and …
opportunity to study human heart development and disease, but they are functionally and …
[HTML][HTML] A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
The methods for the culture and cardiomyocyte differentiation of human embryonic stem
cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex …
cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex …
Cellular and engineered organoids for cardiovascular models
An ensemble of in vitro cardiac tissue models has been developed over the past several
decades to aid our understanding of complex cardiovascular disorders using a reductionist …
decades to aid our understanding of complex cardiovascular disorders using a reductionist …
[HTML][HTML] Heart regeneration: 20 years of progress and renewed optimism
JC Garbern, RT Lee - Developmental cell, 2022 - cell.com
Cardiovascular disease is a leading cause of death worldwide, and thus there remains great
interest in regenerative approaches to treat heart failure. In the past 20 years, the field of …
interest in regenerative approaches to treat heart failure. In the past 20 years, the field of …
[HTML][HTML] Mitochondrial biogenesis, mitochondrial dynamics, and mitophagy in the maturation of cardiomyocytes
Q Ding, Y Qi, SY Tsang - Cells, 2021 - mdpi.com
Pluripotent stem cells (PSCs) can undergo unlimited self-renewal and can differentiate into
all the cell types present in our body, including cardiomyocytes. Therefore, PSCs can be an …
all the cell types present in our body, including cardiomyocytes. Therefore, PSCs can be an …