Discovering functional motifs in long noncoding RNAs

CJ Ross, I Ulitsky - Wiley Interdisciplinary Reviews: RNA, 2022 - Wiley Online Library
Long noncoding RNAs (lncRNAs) are products of pervasive transcription that closely
resemble messenger RNAs on the molecular level, yet function through largely unknown …

Full-length spatial transcriptomics reveals the unexplored isoform diversity of the myocardium post-MI

E Boileau, X Li, IS Naarmann-de Vries, C Becker… - Frontiers in …, 2022 - frontiersin.org
We introduce Single-cell Nanopore Spatial Transcriptomics (scNaST), a software suite to
facilitate the analysis of spatial gene expression from second-and third-generation …

[HTML][HTML] Proteotranscriptomics–A facilitator in omics research

M Levin, F Butter - Computational and Structural Biotechnology Journal, 2022 - Elsevier
Applications in omics research, such as comparative transcriptomics and proteomics,
require the knowledge of the species-specific gene sequence and benefit from a …

Transcriptional atlas analysis from multiple tissues reveals the expression specificity patterns in beef cattle

T Zhang, T Wang, Q Niu, L Xu, Y Chen, X Gao, H Gao… - BMC biology, 2022 - Springer
Background A comprehensive analysis of gene expression profiling across tissues can
provide necessary information for an in-depth understanding of their biological functions. We …

Large-scale multiplexing permits full-length transcriptome annotation of 32 bovine tissues from a single nanopore flow cell

MM Halstead, A Islas-Trejo, DE Goszczynski… - Frontiers in …, 2021 - frontiersin.org
A comprehensive annotation of transcript isoforms in domesticated species is lacking.
Especially considering that transcriptome complexity and splicing patterns are not well …

Nematode gene annotation by machine-learning-assisted proteotranscriptomics enables proteome-wide evolutionary analysis

A Ceron-Noriega, MV Almeida, M Levin… - Genome …, 2023 - genome.cshlp.org
Nematodes encompass more than 24,000 described species, which were discovered in
almost every ecological habitat, and make up> 80% of metazoan taxonomic diversity in …

Identification of protein Isoforms using reference databases built from Long and Short Read RNA-Sequencing

AP Tay, JJ Hamey, GE Martyn… - Journal of Proteome …, 2022 - ACS Publications
Alternative splicing can lead to distinct protein isoforms. These can have different functions
in specific cells and tissues or in different developmental stages. In this study, we explored …

[HTML][HTML] Improved nanopore direct RNA sequencing of cardiac myocyte samples by selective mt-RNA depletion

IS Naarmann-de Vries, J Eschenbach… - Journal of molecular and …, 2022 - Elsevier
RNA sequencing is a powerful tool to analyze gene expression transcriptome wide.
However, RNA sequencing in general and especially the recently developed methods of …

Full-length RNA sequencing provides insights into goldfish evolution under artificial selection

X Du, W Zhang, J Wu, C You, X Dong - International Journal of Molecular …, 2023 - mdpi.com
Goldfish Carassius auratus is an ideal model for exploring fish morphology evolution.
Although genes underlying several ornamental traits have been identified, little is known …

Full-length transcriptome sequencing: an insight into the dog model of heart failure

X Liang, Z Bai, F Wang, Y Han, H Sun… - Frontiers in …, 2021 - frontiersin.org
Heart failure (HF) leads to a progressive increase in morbidity and mortality rates. This study
aimed to explore the transcriptional landscape during HF and identify differentially …