Transcriptional regulation of living materials via extracellular electron transfer

AJ Graham, G Partipilo, CM Dundas… - Nature Chemical …, 2024 - nature.com
Engineered living materials combine the advantages of biological and synthetic systems by
leveraging genetic and metabolic programming to control material-wide properties. Here, we …

Tuning Promoter Strengths for Improved Synthesis and Function of Electron Conduits in Escherichia coli

CP Goldbeck, HM Jensen, MA TerAvest… - ACS synthetic …, 2013 - ACS Publications
Introduction of the electron transfer complex MtrCAB from Shewanella oneidensis MR-1 into
a heterologous host provides a modular and molecularly defined route for electrons to be …

Tuning Extracellular Electron Transfer by Shewanella oneidensis Using Transcriptional Logic Gates

CM Dundas, DJF Walker, BK Keitz - ACS synthetic biology, 2020 - ACS Publications
Extracellular electron transfer (EET) pathways, such as those in the bacterium Shewanella
oneidensis, interface cellular metabolism with a variety of redox-driven applications …

Oxidizing Electrode Potentials Decrease Current Production and Coulombic Efficiency through Cytochrome c Inactivation in Shewanella oneidensis MR‐1

MA TerAvest, LT Angenent - ChemElectroChem, 2014 - Wiley Online Library
Previous transcriptomic profiling of Shewanella oneidensis MR‐1 had suggested that
electron transfer to an anode in a bioelectrochemical system may induce a general stress …

A doubly responsive probe for the detection of Cys4-tagged proteins

N Kotera, E Dubost, G Milanole, E Doris… - Chemical …, 2015 - pubs.rsc.org
Recombinant proteins bearing a tag are crucial tools for assessing protein location or
function. Small tags such as Cys4 tag (tetracysteine; Cys–Cys–X–X–Cys–Cys) are less likely …

Biarsenical fluorescent probes for multifunctional site-specific modification of proteins applicable in life sciences: an overview and future outlook

A Pomorski, A Krężel - Metallomics, 2020 - pubs.rsc.org
Fluorescent modification of proteins of interest (POI) in living cells is desired to study their
behaviour and functions in their natural environment. In a perfect setting it should be easy to …

Shewanella oneidensis and Extracellular Electron Transfer to Metal Oxides

D Saffarini, K Brockman, A Beliaev, R Bouhenni… - Bacteria-metal …, 2015 - Springer
Anaerobic metal reduction by bacteria plays an important role in biogeochemical cycles,
bioremediation, and in biotechnological applications such as electricity generation …

Synthesis of a targeted biarsenical Cy3-Cy5 affinity probe for super-resolution fluorescence imaging

N Fu, Y Xiong, TC Squier - Journal of the American Chemical …, 2012 - ACS Publications
Photoswitchable fluorescent probes capable of the targeted labeling of tagged proteins are
of significant interest due to their ability to enable in situ imaging of protein complexes within …

Synthesis and application of an environmentally insensitive Cy3-based arsenical fluorescent probe to identify adaptive microbial responses involving proximal dithiol …

N Fu, D Su, JR Cort, B Chen, Y Xiong… - Journal of the …, 2013 - ACS Publications
Reversible disulfide oxidation between proximal cysteines in proteins represents a common
regulatory control mechanism to modulate flux through metabolic pathways in response to …

Optimized design and synthesis of a cell-permeable biarsenical cyanine probe for imaging tagged cytosolic bacterial proteins

N Fu, Y Xiong, TC Squier - Bioconjugate Chemistry, 2013 - ACS Publications
To optimize cellular delivery and specific labeling of tagged cytosolic proteins by biarsenical
fluorescent probes built around a cyanine dye (Cy3) scaffold, we have systematically varied …