Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Emerging droplet microfluidics
Droplet microfluidics generates and manipulates discrete droplets through immiscible
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …
Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments
Novel and improved biocatalysts are increasingly sourced from libraries via experimental
screening. The success of such campaigns is crucially dependent on the number of …
screening. The success of such campaigns is crucially dependent on the number of …
Advances in ultrahigh-throughput screening for directed enzyme evolution
U Markel, KD Essani, V Besirlioglu, J Schiffels… - Chemical Society …, 2020 - pubs.rsc.org
Enzymes are versatile catalysts and their synthetic potential has been recognized for a long
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
time. In order to exploit their full potential, enzymes often need to be re-engineered or …
The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation
There is a recognized and growing need for rapid and efficient cell assays, where the size of
microfluidic devices lend themselves to the manipulation of cellular populations down to the …
microfluidic devices lend themselves to the manipulation of cellular populations down to the …
Droplet-based microfluidics in drug discovery, transcriptomics and high-throughput molecular genetics
N Shembekar, C Chaipan, R Utharala, CA Merten - Lab on a Chip, 2016 - pubs.rsc.org
Droplet-based microfluidics enables assays to be carried out at very high throughput (up to
thousands of samples per second) and enables researchers to work with very limited …
thousands of samples per second) and enables researchers to work with very limited …
High-throughput screening by droplet microfluidics: Perspective into key challenges and future prospects
In two decades of development, impressive strides have been made for automating basic
laboratory operations in droplet-based microfluidics, allowing the emergence of a new form …
laboratory operations in droplet-based microfluidics, allowing the emergence of a new form …
Droplet microfluidics—A tool for single‐cell analysis
HN Joensson… - Angewandte Chemie …, 2012 - Wiley Online Library
Droplet microfluidics allows the isolation of single cells and reagents in monodisperse
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …
picoliter liquid capsules and manipulations at a throughput of thousands of droplets per …
Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology
AB Theberge, F Courtois, Y Schaerli… - Angewandte Chemie …, 2010 - Wiley Online Library
Microdroplets in microfluidics offer a great number of opportunities in chemical and
biological research. They provide a compartment in which species or reactions can be …
biological research. They provide a compartment in which species or reactions can be …
Single cell analysis: the new frontier in 'omics'
D Wang, S Bodovitz - Trends in biotechnology, 2010 - cell.com
Cellular heterogeneity that arises from stochastic expression of genes, proteins and
metabolites is a fundamental principle of cell biology, but single cell analysis has been …
metabolites is a fundamental principle of cell biology, but single cell analysis has been …