Decellularized extracellular matrix-based bioinks for engineering tissue-and organ-specific microenvironments

BS Kim, S Das, J Jang, DW Cho - Chemical reviews, 2020 - ACS Publications
Biomaterials-based biofabrication methods have gained much attention in recent years.
Among them, 3D cell printing is a pioneering technology to facilitate the recapitulation of …

[HTML][HTML] Statistical methods for Mendelian randomization in genome-wide association studies: a review

FJ Boehm, X Zhou - Computational and structural biotechnology journal, 2022 - Elsevier
Genome-wide association studies have yielded thousands of associations for many
common diseases and disease-related complex traits. The identified associations made it …

Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry

P Mertins, LC Tang, K Krug, DJ Clark, MA Gritsenko… - Nature protocols, 2018 - nature.com
Here we present an optimized workflow for global proteome and phosphoproteome analysis
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …

Methods to study RNA–protein interactions

M Ramanathan, DF Porter, PA Khavari - Nature methods, 2019 - nature.com
Noncoding RNA sequences, including long noncoding RNAs, small nucleolar RNAs, and
untranslated mRNA regions, accomplish many of their diverse functions through direct …

Detecting RNA modifications in the epitranscriptome: predict and validate

M Helm, Y Motorin - Nature Reviews Genetics, 2017 - nature.com
RNA modifications are emerging players in the field of post-transcriptional regulation of
gene expression, and are attracting a comparable degree of research interest to DNA and …

A review on quantitative multiplexed proteomics

N Pappireddi, L Martin, M Wühr - Chembiochem, 2019 - Wiley Online Library
Over the last few decades, mass spectrometry‐based proteomics has become an
increasingly powerful tool that is now able to routinely detect and quantify thousands of …

[HTML][HTML] Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ

J Cox, MY Hein, CA Luber, I Paron, N Nagaraj… - Molecular & cellular …, 2014 - ASBMB
Protein quantification without isotopic labels has been a long-standing interest in the
proteomics field. However, accurate and robust proteome-wide quantification with label-free …

[HTML][HTML] Systematic analysis of protein turnover in primary cells

T Mathieson, H Franken, J Kosinski, N Kurzawa… - Nature …, 2018 - nature.com
A better understanding of proteostasis in health and disease requires robust methods to
determine protein half-lives. Here we improve the precision and accuracy of peptide ion …

Quantitative mass spectrometry imaging of biological systems

D Unsihuay, D Mesa Sanchez… - Annual review of physical …, 2021 - annualreviews.org
Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed
maps of hundreds of molecules in complex samples with high sensitivity and subcellular …