Decellularized extracellular matrix-based bioinks for engineering tissue-and organ-specific microenvironments
Biomaterials-based biofabrication methods have gained much attention in recent years.
Among them, 3D cell printing is a pioneering technology to facilitate the recapitulation of …
Among them, 3D cell printing is a pioneering technology to facilitate the recapitulation of …
[HTML][HTML] Statistical methods for Mendelian randomization in genome-wide association studies: a review
Genome-wide association studies have yielded thousands of associations for many
common diseases and disease-related complex traits. The identified associations made it …
common diseases and disease-related complex traits. The identified associations made it …
Guidelines for the use of flow cytometry and cell sorting in immunological studies
A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …
immunology and flow cytometry community. They provide the theory and key practical …
Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry
Here we present an optimized workflow for global proteome and phosphoproteome analysis
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …
of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed …
Methods to study RNA–protein interactions
Noncoding RNA sequences, including long noncoding RNAs, small nucleolar RNAs, and
untranslated mRNA regions, accomplish many of their diverse functions through direct …
untranslated mRNA regions, accomplish many of their diverse functions through direct …
Detecting RNA modifications in the epitranscriptome: predict and validate
RNA modifications are emerging players in the field of post-transcriptional regulation of
gene expression, and are attracting a comparable degree of research interest to DNA and …
gene expression, and are attracting a comparable degree of research interest to DNA and …
A review on quantitative multiplexed proteomics
N Pappireddi, L Martin, M Wühr - Chembiochem, 2019 - Wiley Online Library
Over the last few decades, mass spectrometry‐based proteomics has become an
increasingly powerful tool that is now able to routinely detect and quantify thousands of …
increasingly powerful tool that is now able to routinely detect and quantify thousands of …
[HTML][HTML] Accurate proteome-wide label-free quantification by delayed normalization and maximal peptide ratio extraction, termed MaxLFQ
Protein quantification without isotopic labels has been a long-standing interest in the
proteomics field. However, accurate and robust proteome-wide quantification with label-free …
proteomics field. However, accurate and robust proteome-wide quantification with label-free …
[HTML][HTML] Systematic analysis of protein turnover in primary cells
A better understanding of proteostasis in health and disease requires robust methods to
determine protein half-lives. Here we improve the precision and accuracy of peptide ion …
determine protein half-lives. Here we improve the precision and accuracy of peptide ion …
Quantitative mass spectrometry imaging of biological systems
D Unsihuay, D Mesa Sanchez… - Annual review of physical …, 2021 - annualreviews.org
Mass spectrometry imaging (MSI) is a powerful, label-free technique that provides detailed
maps of hundreds of molecules in complex samples with high sensitivity and subcellular …
maps of hundreds of molecules in complex samples with high sensitivity and subcellular …