Antibody-gold nanoparticle bioconjugates for biosensors: synthesis, characterization and selected applications

L Zhang, Y Mazouzi, M Salmain, B Liedberg… - Biosensors and …, 2020 - Elsevier
Abstract Antibody-Gold nanoparticle (Ab-AuNP) bioconjugates are widely used in the field of
biosensing. This prompted researchers to set up various strategies to conjugate antibodies …

AuNPs as an important inorganic nanoparticle applied in drug carrier systems

W Li, Z Cao, R Liu, L Liu, H Li, X Li, Y Chen… - Artificial cells …, 2019 - Taylor & Francis
Gold nanoparticles (AuNPs), as a kind of inorganic nanoparticle, have been gradually
recognized as one of the most promising nanomaterials, which is attributed to their unique …

pH impacts the orientation of antibody adsorbed onto gold nanoparticles

G Ruiz, K Tripathi, S Okyem, JD Driskell - Bioconjugate chemistry, 2019 - ACS Publications
Novel detection strategies that exploit the unique properties of gold nanoparticles (AuNPs)
hold great promise for the advancement of diagnostic testing. Fundamental to many of these …

Quantifying bound and active antibodies conjugated to gold nanoparticles: A comprehensive and robust approach to evaluate immobilization chemistry

K Tripathi, JD Driskell - ACS omega, 2018 - ACS Publications
Gold nanoparticles (AuNPs) functionalized with antibodies have the potential to improve
biosensing technology because of the unique optical properties of AuNPs and the specificity …

[HTML][HTML] Nanoparticle-based biosensors for detection of extracellular vesicles in liquid biopsies

B Martín-Gracia, A Martín-Barreiro… - Journal of Materials …, 2020 - pubs.rsc.org
Tumor-derived extracellular vesicles have emerged as an alternative source of cancer
biomarkers in liquid biopsies. Despite their clinical potential, traditional methods for isolation …

Site-specific conjugation of an antibody on a gold nanoparticle surface for one-step diagnosis of prostate specific antigen with dynamic light scattering

N Mustafaoglu, T Kiziltepe, B Bilgicer - Nanoscale, 2017 - pubs.rsc.org
Small dimensions of gold nanoparticles (AuNPs) necessitate antibodies to be immobilized in
an oriented fashion in order to conserve their antigen binding activity for proper function. In …

Direct quantification of surface coverage of antibody in IgG-Gold nanoparticles conjugates

L Zhang, D Hu, M Salmain, B Liedberg, S Boujday - Talanta, 2019 - Elsevier
It is of paramount importance to be able to accurately quantify surface coverage of
antibodies on gold nanoparticles (AuNP) so as to optimise the sensitivity of AuNP-based …

Antibody conjugated green synthesized chitosan-gold nanoparticles for optical biosensing

H Majdi, R Salehi, M Pourhassan-Moghaddam… - Colloid and Interface …, 2019 - Elsevier
In this study a novel antibody conjugated chitosan-gold nanoparticles (Ab-CS-GNPs) was
engineered by the green synthesized method for detection of the target antigen (Ag) by three …

[HTML][HTML] Rapid and easy-to-use method for accurate characterization of target binding and kinetics of magnetic particle bioconjugates for biosensing

AV Pushkarev, AV Orlov, SL Znoyko, VA Bragina… - Sensors, 2021 - mdpi.com
The ever-increasing use of magnetic particle bioconjugates (MPB) in biosensors calls for
methods of comprehensive characterization of their interaction with targets. Label-free …

[HTML][HTML] Purification of Crimean–Congo hemorrhagic fever virus nucleoprotein and its utility for serological diagnosis

BP Lombe, H Miyamoto, T Saito, R Yoshida… - Scientific reports, 2021 - nature.com
Crimean–Congo hemorrhagic fever virus (CCHFV) causes a zoonotic disease, Crimean–
Congo hemorrhagic fever (CCHF) endemic in Africa, Asia, the Middle East, and …