Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Climate change gives rise to numerous environmental stresses, including soil salinity.
Salinity/salt stress is the second biggest abiotic factor affecting agricultural productivity …

Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …

Cell–cell interactions orchestrate organismal development, homeostasis and single-cell
functions. When cells do not properly interact or improperly decode molecular messages …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

In chemical biology research, various fluorescent probes have been developed and used to
visualize target proteins or molecules in living cells and tissues, yet there are limitations to …

Gene therapy would benefit from a miniature CRISPR system that fits into the small adeno-
associated virus (AAV) genome and has high cleavage activity and specificity in eukaryotic …

Clustered regularly interspaced short palindromic repeat (CRISPR) and their associated
protein (Cas-9) is the most effective, efficient, and accurate method of genome editing tool in …

Huntington disease (HD) is a neurodegenerative disease caused by CAG repeat expansion
in the huntingtin gene (HTT) and involves a complex web of pathogenic mechanisms …

Throughout their evolutionary history, bacteria have faced diverse threats from other
microorganisms, including competing bacteria, bacteriophages and predators. In response …