Abnormal gene expression level or expression of genes containing deleterious mutations
are two of the main determinants which lead to genetic disease. To obtain a therapeutic …

CRISPR-mediated genome editing of primary human lymphocytes is typically carried out via
electroporation, which can be cytotoxic, cumbersome and costly. Here we show that the …

Regulatory T (Treg) cells are critical for tolerance to self-antigens and for preventing
autoimmunity. Foxp3 has been identified as a Treg cell lineage-defining transcription factor …

Genetic modification of specific genes is emerging as a useful tool to enhance the functions
of antitumor T cells in adoptive immunotherapy. Current advances in CRISPR/Cas9 …

The use of a patient's own immune or tumor cells, manipulated ex vivo, enables Ag-or
patient-specific immunotherapy. Despite some clinical successes, there remain significant …

Cancer has become one of the common causes of mortality around the globe due to
mutations in the genome which allows rapid growth of cells uncontrollably without repairing …

In CRISPR/Cas9 genome editing, the tight and persistent target binding of Cas9 provides an
opportunity for efficient genetic and epigenetic modification on genome. In particular …

The broad effectiveness of T cell-based therapy for treating solid tumour cancers remains
limited. This is partly due to the growing appreciation that immune cells must inhabit and …

Chimeric antigen receptor (CAR) T cell therapy is a promising cancer treatment modality.
The breakthroughs in CAR T cell therapy were, in part, possible with the help of cell analysis …

Induced pluripotent stem cell (iPSC)-derived T (iT) cells represent a groundbreaking frontier
in adoptive cell therapies with engineered T cells, poised to overcome pivotal limitations …