Droplet-based microfluidics

T Moragues, D Arguijo, T Beneyton, C Modavi… - Nature Reviews …, 2023 - nature.com
Droplet-based microfluidic systems generate, manipulate and control sub-microlitre droplets
enclosed within an immiscible carrier fluid. Owing to a number of remarkable features, such …

Recent advances in droplet microfluidics for single-cell analysis

Z Jiang, H Shi, X Tang, J Qin - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
Single-cell manipulation and analysis is essential for studying many fundamental biological
processes and discovering cellular heterogeneity. Multi-parameter studies of intercellular …

Single-cell RNA-seq methods to interrogate virus-host interactions

K Ratnasiri, AJ Wilk, MJ Lee, P Khatri… - Seminars in …, 2023 - Springer
The twenty-first century has seen the emergence of many epidemic and pandemic viruses,
with the most recent being the SARS-CoV-2-driven COVID-19 pandemic. As obligate …

Microfluidics for neuronal cell and circuit engineering

R Habibey, JE Rojo Arias, J Striebel… - Chemical …, 2022 - ACS Publications
The widespread adoption of microfluidic devices among the neuroscience and neurobiology
communities has enabled addressing a broad range of questions at the molecular, cellular …

Deciphering the biology of circulating tumor cells through single-cell RNA sequencing: implications for precision medicine in cancer

S Orrapin, P Thongkumkoon, S Udomruk… - International Journal of …, 2023 - mdpi.com
Circulating tumor cells (CTCs) hold unique biological characteristics that directly involve
them in hematogenous dissemination. Studying CTCs systematically is technically …

Recent advances in microfluidic single-cell analysis and its applications in drug development

Y Jiao, L Gao, Y Ji, W Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
Cellular heterogeneity is prevalent in all cell populations. Single-cell analysis (SCA) could
unveil the heterogeneity and thus gain unique insights into complex biological processes …

Back to basics: a simplified improvement to multiple displacement amplification for microbial single-cell genomics

MS Sobol, AK Kaster - International Journal of Molecular Sciences, 2023 - mdpi.com
Microbial single-cell genomics (SCG) provides access to the genomes of rare and
uncultured microorganisms and is a complementary method to metagenomics. Due to the …

Microsystem advances through integration with artificial intelligence

HF Tsai, S Podder, PY Chen - Micromachines, 2023 - mdpi.com
Microfluidics is a rapidly growing discipline that involves studying and manipulating fluids at
reduced length scale and volume, typically on the scale of micro-or nanoliters. Under the …

Droplets microfluidics platform—A tool for single cell research

B Li, X Ma, J Cheng, T Tian, J Guo, Y Wang… - … in Bioengineering and …, 2023 - frontiersin.org
Cells are the most basic structural and functional units of living organisms. Studies of cell
growth, differentiation, apoptosis, and cell-cell interactions can help scientists understand …

Single-cell RNA sequencing technology landscape in 2023

HQ Qu, C Kao, H Hakonarson - Stem Cells, 2024 - academic.oup.com
Single-cell RNA sequencing (scRNA-seq) has revolutionized our understanding of cellular
heterogeneity and the dynamics of gene expression, bearing profound significance in stem …