Lentiviral vectors (LV) have seen considerably increase in use as gene therapy vectors for
the treatment of acquired and inherited diseases. This review presents the state of the art of …

The clinical application of self-inactivating (SIN) retroviral vectors requires an efficient vector
production technology. To enable production of γ-retroviral SIN vectors from stable producer …

This article describes a novel method merging the cloning of viral vector producer cells with
vector titer screening, allowing for screening 200–500 clones in 2 weeks. It makes use of a …

Hepatitis C pseudoparticles (HCVpp) are used to evaluate HCV cell entry while screening
for neutralizing antibodies induced upon vaccination or while screening for new antiviral …

Gammaretrovirus and lentivirus are the preferred viral vectors to genetically modify T and
natural killer cells to be used in immune cell therapies. The transduction efficiency of …

The majority of processes to manufacture biotherapeutics for use in humans are based on
mammalian cell lines. Established cell lines are modified by recombinant DNA technologies …

The clinical application of self-inactivating (SIN) retroviral vectors requires an efficient vector
production technology. To enable production of γ-retroviral SIN-vectors from stable producer …

One of the major limitations of retroviral vectors is based on the inability to produce, purify
and concentrate high titers of infective virus, as required for gene therapy clinical …

After the creation of the Biosafety platform most of the safety issues span-off but vector
design and production bridged these two platforms with clear positive results. Regarding the …

In this chapter, we give an overview on the chromosomal elements that have a dominant
influence on the transcriptional activity of transgenes upon integration.(For other cellular …