[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
Engineering extracellular electron transfer pathways of electroactive microorganisms by synthetic biology for energy and chemicals production
J Zhang, F Li, D Liu, Q Liu, H Song - Chemical Society Reviews, 2024 - pubs.rsc.org
The excessive consumption of fossil fuels causes massive emission of CO2, leading to
climate deterioration and environmental pollution. The development of substitutes and …
climate deterioration and environmental pollution. The development of substitutes and …
[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …
technologies, developed over the past 20 years, have enormously accelerated the …
[HTML][HTML] Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation
Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …
produced from CO 2—but formatotrophy has been engineered in only a few industrially …
Towards next-generation cell factories by rational genome-scale engineering
Metabolic engineering holds the promise to transform the chemical industry and to support
the transition into a circular bioeconomy, by engineering cellular biocatalysts that efficiently …
the transition into a circular bioeconomy, by engineering cellular biocatalysts that efficiently …
An expanded CRISPR–Cas9-assisted recombineering toolkit for engineering genetically intractable Pseudomonas aeruginosa isolates
D Pankratz, NO Gomez, A Nielsen, A Mustafayeva… - Nature protocols, 2023 - nature.com
Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …
engineering procedures. Since their inception (more than 30 years ago), methods based …
[HTML][HTML] A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes
Acetyl-coenzyme A (AcCoA) is a metabolic hub in virtually all living cells, serving as both a
key precursor of essential biomass components and a metabolic sink for catabolic pathways …
key precursor of essential biomass components and a metabolic sink for catabolic pathways …
[HTML][HTML] Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration
Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …
bioproduction. These properties can be introduced using various enzyme engineering …
Highly Efficient Biosynthesis of Protocatechuic Acid via Recombinant Pseudomonas putida KT2440
J Li, J Fu, C Yue, Y Shang, BC Ye - Journal of Agricultural and …, 2023 - ACS Publications
Owing to their physiological activities, plant-derived phenolic acids, such as protocatechuic
acid (PCA), have extensive applications and market prospects. However, traditional …
acid (PCA), have extensive applications and market prospects. However, traditional …
Systems analysis of highly multiplexed CRISPR-base editing in streptomycetes
CRISPR tools, especially Cas9n-sgRNA guided cytidine deaminase base editors such as
CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One …
CRISPR-BEST, have dramatically simplified genetic manipulation of streptomycetes. One …