Genetically modified animals are vital for gaining a proper understanding of disease
mechanisms. Mice have long been the mainstay of basic research into a wide variety of …

The human population is growing, and as a result we need to produce more food whilst
reducing the impact of farming on the environment. Selective breeding and genomic …

Zebrafish are an established research organism that has made many contributions to our
understanding of vertebrate tissue and organ development, yet there are still significant …

In 2008, a consortium led by the Agricultural Research Service (ARS) and the National
Institute for Food and Agriculture (NIFA) published the “Blueprint for USDA Efforts in …

Genetic improvement of cattle around the globe has been, and will continue to be, an
important driver of animal agriculture sustainability. There are several reproductive and …

Sheep and goats are valuable livestock species that have been raised for their production of
meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period …

Spermatogonial stem cell transplantation (SSCT) is an experimental technique for transfer of
germline between donor and recipient males that could be used as a tool for biomedical …

Accelerated development of novel CRISPR/Cas9-based genome editing techniques
provides a feasible approach to introduce a variety of precise modifications in the …

Precise genome editing of large animals applied to livestock and biomedicine is nowadays
possible since the CRISPR revolution. This review summarizes the latest advances and the …

Contents In the last decade, the research community has witnessed a blooming of targeted
genome editing tools and applications. Novel programmable DNA nucleases such as zinc …