Oligonucleotide Fluorescence In Situ Hybridization: An Efficient Chromosome Painting Method in Plants

A Harun, H Liu, S Song, S Asghar, X Wen, Z Fang… - Plants, 2023 - mdpi.com
Fluorescence in situ hybridization (FISH) is an indispensable technique for studying
chromosomes in plants. However, traditional FISH methods, such as BAC, rDNA, tandem …

Comparative Analysis of Chloroplast Genome in Saccharum spp. and Related Members of 'Saccharum Complex'

S Li, W Duan, J Zhao, Y Jing, M Feng, B Kuang… - International Journal of …, 2022 - mdpi.com
High ploids of the sugarcane nuclear genome limit its genomic studies, whereas its
chloroplast genome is small and conserved, which is suitable for phylogenetic studies and …

Oligo-barcode illuminates holocentric karyotype evolution in Rhynchospora (Cyperaceae)

Y Mata-Sucre, LM Parteka, CM Ritz… - Frontiers in Plant …, 2024 - frontiersin.org
Holocentric karyotypes are assumed to rapidly evolve through chromosome fusions and
fissions due to the diffuse nature of their centromeres. Here, we took advantage of the recent …

Molecular Dissection of the 5S Ribosomal RNA-Intergenic Transcribed Spacers in Saccharum spp. and Tripidium spp.

YB Pan, JR Todd, L Lomax, PM White Jr, SA Simpson… - Agronomy, 2023 - mdpi.com
Due to complex polyploid, sugarcane whole genome sequencing and characterization lag
far behind other crops. PCR-based DNA markers are a viable low-cost option to evaluate …

Accurate Chromosome Identification in the Prunus Subgenus Cerasus (Prunus pseudocerasus) and its Relatives by Oligo-FISH

L Wang, Y Feng, Y Wang, J Zhang, Q Chen… - International Journal of …, 2022 - mdpi.com
A precise, rapid and straightforward approach to chromosome identification is fundamental
for cytogenetics studies. However, the identification of individual chromosomes was not …

多荧光标记引物增强甘蔗染色体寡聚核苷酸探针杂交信号

李心怡, 姜春秀, 薛丽, 蒋洪涛, 姚伟, 邓祖湖… - 生物技术 …, 2023 - biotech.aiijournal.com
荧光原位杂交技术(fluorescence in situ hybridization, FISH) 是植物分子细胞遗传学研究最为
重要的手段之一. 近些年, 基于参考基因组设计的低拷贝寡聚核苷酸探针在FISH …

Enhancing Hybridization Signal of Sugarcane Chromosome Oligonucleotide Probe via Multiple Fluorescence Labeled Primers

X LI, C JIANG, L XUE, H JIANG, W YAO… - Biotechnology …, 2023 - biotech.aiijournal.com
Fluorescence in situ hybridization (FISH) is one of the most important methods in plant
molecular cytogenetics. In recent years, low copy oligonucleotide probes based on …

Cytomolecular Organisation of the Nuclear Genome

R Hasterok, N Borowska-Zuchowska… - International Journal of …, 2022 - mdpi.com
Modern molecular cytogenetics allows many aspects of the nuclear genome structure,
function, and evolution to be analysed within the topographic context of mitotic and meiotic …