Drug-induced nephrotoxicity assessment in 3D cellular models

P Yu, Z Duan, S Liu, I Pachon, J Ma, GP Hemstreet… - Micromachines, 2021 - mdpi.com
The kidneys are often involved in adverse effects and toxicity caused by exposure to foreign
compounds, chemicals, and drugs. Early predictions of these influences are essential to …

An analysis modality for vascular structures combining tissue-clearing technology and topological data analysis

K Takahashi, K Abe, SI Kubota, N Fukatsu… - Nature …, 2022 - nature.com
The blood and lymphatic vasculature networks are not yet fully understood even in mouse
because of the inherent limitations of imaging systems and quantification methods. This …

Rapid and fully automated blood vasculature analysis in 3D light-sheet image volumes of different organs

P Spangenberg, N Hagemann, A Squire, N Förster… - Cell Reports …, 2023 - cell.com
Light-sheet fluorescence microscopy (LSFM) can produce high-resolution tomograms of
tissue vasculature with high accuracy. However, data processing and analysis is laborious …

Therapy-induced modulation of tumor vasculature and oxygenation in a murine glioblastoma model quantified by deep learning-based feature extraction

N Bauer, D Beckmann, D Reinhardt, N Frost, S Bobe… - Scientific Reports, 2024 - nature.com
Glioblastoma presents characteristically with an exuberant, poorly functional vasculature
that causes malperfusion, hypoxia and necrosis. Despite limited clinical efficacy, anti …

Multimodal imaging of target detection algorithm under artificial intelligence in the diagnosis of early breast cancer

M Jiang, S Lei, J Zhang, L Hou… - Journal of Healthcare …, 2022 - Wiley Online Library
This study aimed to analyze the diagnostic value of multimodal images based on artificial
intelligence target detection algorithms for early breast cancer, so as to provide help for …

[HTML][HTML] Tissue clearing method in visualization of cancer progression and metastasis

K Takahashi-Yamashiro, K Miyazono - Upsala Journal of Medical …, 2024 - ncbi.nlm.nih.gov
Since various imaging modalities have been developed, cancer metastasis can be detected
from an early stage. However, limitations still exist, especially in terms of spatial resolution …

Effect of methanol fixation on single-cell RNA sequencing of the murine dentate gyrus

M Sánchez-Carbonell, P Jiménez Peinado… - Frontiers in Molecular …, 2023 - frontiersin.org
Single-cell RNA sequencing (scRNA-seq) provides a powerful tool to evaluate the
transcriptomic landscape and heterogeneity of thousands of cells in parallel. However …

Tissue clearing may alter emission and absorption properties of common fluorophores

F Eliat, R Sohn, H Renner, T Kagermeier, S Volkery… - Scientific Reports, 2022 - nature.com
In recent years, 3D cell culture has been gaining a more widespread following across many
fields of biology. Tissue clearing enables optical analysis of intact 3D samples and …

Hierarchical random walker segmentation for large volumetric biomedical images

D Drees, F Eilers, X Jiang - IEEE Transactions on Image …, 2022 - ieeexplore.ieee.org
The random walker method for image segmentation is a popular tool for semi-automatic
image segmentation, especially in the biomedical field. However, its linear asymptotic run …

Vegfr3-tdTomato, a reporter mouse for microscopic visualization of lymphatic vessel by multiple modalities

E Redder, N Kirschnick, S Bobe, R Hägerling… - PLoS …, 2021 - journals.plos.org
Lymphatic vessels are indispensable for tissue fluid homeostasis, transport of solutes and
dietary lipids and immune cell trafficking. In contrast to blood vessels, which are easily …