Effective metabolic engineering of microorganisms relies on balanced expression of both
heterologous and endogenous genes to channel metabolic flux towards products of interest …

Unnatural gene expression imposes a load on engineered microorganisms which
decreases their growth and subsequent production yields, a phenomenon known as burden …

Chemicals production by engineered microorganisms often requires induction of target gene
expression at an appropriate cell density to reduce conflict with cell growth. The lux system …

Low-molecular-weight hyaluronan (LMW-HA) has attracted much attention because of its
many potential applications. Here, we efficiently produced specific LMW-HAs from sucrose …

Programmable control over an addressable global regulator would enable simultaneous
repression of multiple genes and would have tremendous impact on the field of synthetic …

In vivo biosensors that can convert metabolite concentrations into measurable output signals
are valuable tools for high-throughput screening and dynamic pathway control in the field of …

Writing artificial logic and dynamic function into complex cellular background to achieve
desired phenotypes or improved outputs calls for the development of new genetic tools as …

Metabolic engineering entails target modification of cell metabolism to maximize cell's
production potential. Due to the complexity of cell metabolism, feedback genetic circuits …

Highlights•Microbial production of valuable chemicals can be improved by dynamic
regulation.•Dynamic regulation balances flux between growth of cell and production of …

Food is essential to provide energy for human cellular metabolism, and is usually made from
plants or animals. Beside plants and animals, other important food sources are made by …