Silencing of the 20S proteasomal subunit-α6 triggers full oogenesis arrest and increased mRNA levels of the selective autophagy adaptor protein p62/SQSTM1 in the …

A Faria-Reis, S Santos-Araújo, J Pereira… - PLOS Neglected …, 2023 - journals.plos.org
The high reproductive rates of insects contribute significantly to their ability to act as vectors
of a variety of vector-borne diseases. Therefore, it is strategically critical to find molecular …

Determination of internal controls for quantitative gene expression of Spodoptera litura under microbial pesticide stress

S Wu, Y Luo, Z Zeng, Y Yu, S Zhang, Y Hu, L Chen - Scientific Reports, 2024 - nature.com
Quantitative real-time polymerase chain reaction (qRT-PCR) has become a commonly used
method for the quantification of gene expression. However, accurate qRT-PCR analysis …

Deficiency of Brummer lipase disturbs lipid mobilization and locomotion, and impairs reproduction due to defects in the eggshell ultrastructure in the insect vector …

DS Arêdes, T Rios, LF Carvalho-Kelly, V Braz… - … et Biophysica Acta (BBA …, 2024 - Elsevier
Rhodnius prolixus is a hematophagous insect, which feeds on large and infrequent blood
meals, and is a vector of trypanosomatids that cause Chagas disease. After feeding, lipids …

Multiplex real-time PCR for the detection of insect DNA and determination of contents of Tenebrio molitor, Locusta migratoria and Achaeta domestica in food

R Köppel, R Schum, M Habermacher, C Sester… - … Food Research and …, 2019 - Springer
In Europe, edible insects are not a part of a common diet. But according to the Food and
Agriculture Organization of the united nations (FAO), animal protein from insects could be an …

Gene identification and RNAi-silencing of p62/SQSTM1 in the vector Rhodnius prolixus reveals a high degree of sequence conservation but no apparent deficiency …

J Pereira, S Santos-Araujo, L Bomfim, KC Gondim… - Plos one, 2023 - journals.plos.org
Autophagy and the ubiquitin-proteasome system (UPS) are important cellular mechanisms
that coordinate protein degradation essential for proteostasis. P62/SQSTM1 is a receptor …

The ACBP gene family in Rhodnius prolixus: Expression, characterization and function of RpACBP-1

D Majerowicz, HK Hannibal-Bach, RSC Castro… - Insect biochemistry and …, 2016 - Elsevier
The acyl-CoA-binding proteins (ACBP) constitute a family of conserved proteins that bind
acyl-CoA with high affinity and protect it from hydrolysis. Thus, ACBPs may have essential …

GENE IDENTIFICATION AND ENZYMATIC PROPERTIES OF A MEMBRANE‐BOUND TREHALASE FROM THE OVARY OF R HODNIUS PROLIXUS

R Santos, M Alves‐Bezerra… - Archives of insect …, 2012 - Wiley Online Library
Trehalose represents the main hemolymph sugar in most insects and its metabolic
availability is regulated by trehalase. In this study, trehalase activity associated with the …

Lipid metabolism in Rhodnius prolixus: lessons from the genome

D Majerowicz, GM Calderón-Fernández… - Gene, 2017 - Elsevier
The kissing bug Rhodnius prolixus is both an important vector of Chagas' disease and an
interesting model for investigation into the field of physiology, including lipid metabolism …

The neuropeptide CCHamide2 regulates diuresis in the Chagas disease vector Rhodnius prolixus

N Capriotti, JP Ianowski, P Gioino… - Journal of Experimental …, 2019 - journals.biologists.com
Given that hematophagous insects ingest large quantities of blood in a single meal, they
must undergo a rapid post-prandial diuresis in order to maintain homeostasis. In the kissing …

Selection of suitable reference genes for normalization of quantitative real-time PCR in cartilage tissue injury and repair in rabbits

XX Peng, RL Zhao, W Song, HR Chu, M Li… - International journal of …, 2012 - mdpi.com
When studying the altered expression of genes associated with cartilage regeneration by
quantitative real-time RT-PCR (RT-qPCR), reference genes with highly stable expression …