Oxygen‐tolerant [NiFe] hydrogenases are attractive biocatalysts for utilization in H2/O2 fuel
cells, which thereby reduces the amount of platinum‐based catalysts. The O2‐tolerant …

Hydrogen metabolism is restricted to three classes of enzymes,[NiFe],[FeFe], and [Fe]
hydrogenases.[1] As H2 oxidation catalysts,[NiFe] hydrogenases are competitive with the …

Two complementary experimental approaches were utilized to examine the extent to which
free soil hydrogenases and H2-oxidizing bacteria contribute to the soil uptake of …

A suite of dynamic electrochemical techniques known as protein film electrochemistry (PFE)
offers important insight into the roles of active sites in enzymes, including properties of …

The membrane-bound [NiFe]-hydrogenase of Cupriavidus necator is a rare example of a
truly O2-tolerant hydrogenase. It catalyzes the oxidation of H2 into 2e− and 2H+ in the …

The catalytic properties of hydrogenases are nature's answer to the seemingly simple
reaction H2⇌ 2H++ 2e–. Members of the phylogenetically diverse subgroup of [NiFe] …

Many but not all iron–sulphur clusters in metalloproteins are known to be sensitive to
molecular oxygen with dramatic consequences for their biological function. We performed a …

Biocatalysts that mediate the H 2-dependent reduction of NAD+ to NADH are attractive from
both a fundamental and applied perspective. Here we present the first biochemical and …

[NiFe]-hydrogenases catalyze the cleavage of molecular hydrogen into protons and
electrons and represent promising tools for H2-based technologies such as biofuel cells …

We report the successful increase in performance of a fuel cell based on organometallic
catalysis. An organometallic [NiIIRuIV] peroxo complex functions as cathode catalyst and …