This edited book is written for students, postdocs and established investigators who want to
enter the field of single-particle cryo-EM. This is a recently developed method to determine …

Protein functions are temperature-dependent, but protein structures are usually solved at a
single (often low) temperature because of limitations on the conditions of crystal growth or …

Twinkle is a mitochondrial replicative helicase which can self-load onto and unwind
mitochondrial DNA. Nearly 60 mutations on Twinkle have been linked to human …

DNA polymerase θ (Pol θ) plays an essential role in the microhomology-mediated end
joining (MMEJ) pathway for repairing DNA double-strand breaks. However, the mechanisms …

The β subunit of the Escherichia coli replicative DNA polymerase III holoenzyme is the
sliding clamp that interacts with the α (polymerase) subunit to maintain the high processivity …

A ring-shaped helicase unwinds DNA during chromosome replication in all organisms.
Replicative helicases generally unwind duplex DNA an order of magnitude slower …

DNA replication mechanisms are conserved across all organisms. The proteins required to
initiate, coordinate, and complete the replication process are best characterized in model …

Twinkle is the ring-shaped replicative helicase within the human mitochondria with high
homology to bacteriophage T7 gp4 helicase–primase. Unlike many orthologs of Twinkle, the …

Twin CMG complexes are assembled head-to-head around duplex DNA at eukaryotic
origins of replication. Mcm10 activates CMGs to form helicases that encircle single-strand …

The eukaryotic replisome coordinates template unwinding and nascent-strand synthesis to
drive DNA replication fork progression and complete efficient genome duplication. During its …