Resistance threatens to render antibiotics—which are essential for modern medicine—
ineffective, thus posing a threat to human health. The discovery of novel classes of …

Bacterial pathogens require the iron-containing cofactor heme to cause disease. Heme is
essential to the function of hemoproteins, which are involved in energy generation by the …

Opportunistic infections caused by Pseudomonas aeruginosa can be acute or chronic. While
acute infections often spread rapidly and can cause tissue damage and sepsis with high …

Genomics offered the promise of transforming antibiotic discovery by revealing many new
essential genes as good targets, but the results fell short of the promise. While numerous …

Transposon insertion sequencing (TIS) is a powerful approach that can be extensively
applied to the genome-wide definition of loci that are required for bacterial growth under …

Pseudomonas aeruginosa is a versatile opportunistic pathogen capable of infecting a broad
range of hosts, in addition to thriving in a broad range of environmental conditions outside of …

Acinetobacter baumannii is a Gram-negative bacterium that causes diseases such as
pneumonia, bacteremia, and soft tissue infections in hospitalized patients. Relatively little is …

Driven in part by its metabolic versatility, high intrinsic antibiotic resistance, and a large
repertoire of virulence factors, Pseudomonas aeruginosa is expertly adapted to thrive in a …

The essential functions of a bacterial pathogen reflect the most basic processes required for
its viability and growth, and represent potential therapeutic targets. Most screens for …

Cells regularly experience fluid flow in natural systems. However, most experimental
systems rely on batch cell culture and fail to consider the effect of flow-driven dynamics on …