The secretory production of recombinant proteins by the Gram-negative bacterium
Escherichia coli has several advantages over intracellular production as inclusion bodies. In …

Bacterial inclusion bodies (IBs) have long been considered as inactive, unfolded waste
material produced by heterologous overexpression of recombinant genes. In industrial …

Background Conjugate vaccines in which polysaccharide antigens are covalently linked to
carrier proteins belong to the most effective and safest vaccines against bacterial pathogens …

Horseradish and soybean peroxidases (HRP and SBP, respectively) are useful
biotechnological tools. HRP is often termed the classical plant heme peroxidase and …

Protein production under the control of lac operon regulatory elements using autoinduction
is based on diauxic growth of Escherichia coli on lactose after consumption of more …

Background Thermostable lipases from microbial sources have been substantially
overexpressed in E. coli, however, these enzymes are often produced with low-level …

Background Recombinant protein production can be stressful to the host organism. The
extent of stress is determined by the specific properties of the recombinant transcript and …

Slow protein release from amyloidal materials is a molecular platform used by nature to
control protein hormone secretion in the endocrine system. The molecular mechanics of the …

A comparison of the metabolic response of Escherichia coli BL21 (DE3) towards the
production of human basic fibroblast growth factor (hFGF‐2) or towards carbon overfeeding …

In this study, we compared basic expression approaches for the efficient expression of
bioactive recombinant human interleukin-6 (IL6), as an example for a difficult-to-express …