State-of-the-art strain engineering techniques for the methylotrophic yeast Pichia pastoris
(syn. Komagataella spp.) include overexpression of endogenous and heterologous genes …

A highly effective metabolic pathway is the key for an efficient cell factory. However, the
engineered homologous or heterologous multi-gene pathway may be unbalanced …

Standard approaches for dCas9-based modification of gene expression are limited in the
ability to multiplex targets, establish streamlined cassettes, and utilize commonly studied Pol …

Saccharomyces cerevisiae is an important model eukaryotic microorganism and widely
applied in fundamental research and the production of various chemicals. Its ability to …

Fungal species have the capability of producing an overwhelming diversity of bioactive
substances that can have beneficial but also detrimental effects on human health. These so …

The emergence of CRISPR-Cas9 for targeted genome editing and regulation has enabled
the manipulation of desired traits and enhanced strain development of nonmodel …

Cas9, a CRISPR-associated RNA-guided nuclease, has been rapidly adopted as a tool for
biochemical and genetic manipulation of DNA. Although complexes between Cas9 and …

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas nuclease
system is a powerful tool for genome editing, and its simple programmability has enabled …

Key to our ability to increase recombinant protein production through secretion is a better
understanding of the pathways that interact to translate, process and export mature proteins …

Background The construction of microbial cell factories requires cost-effective and rapid
strain development through metabolic engineering. Recently, RNA-guided CRISPR …