Deciphering gene function is fundamental to engineering of microbiology. The clustered
regularly interspaced short palindromic repeats (CRISPR) system has been adapted for …

The yeast Candida glabrata is an emerging, often drug-resistant opportunistic human
pathogen that can cause severe systemic infections in immunocompromised individuals. At …

The CRISPR/Cas9 systems have been developed as tools for genetic engineering and
metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 …

The low costs of array‐synthesized oligonucleotide libraries are empowering rapid
advances in quantitative and synthetic biology. However, high synthesis error rates, uneven …

An essential gene encodes for a cellular function that is required for viability. Although
viability is a straightforward phenotype to analyze in yeast, defining a gene as essential is …

Abstract The engineering of Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR)-CRISPR-associated proteins continues to expand the toolkit available for genome …

Control of biological populations is an ongoing challenge in many fields, including
agriculture, biodiversity, ecological preservation, pest control, and the spread of disease. In …

For the fungal pathogen Candida albicans, genetic overexpression readily occurs via a
diversity of genomic alterations, such as aneuploidy and gain-of-function mutations, with …

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-
associated (Cas) immune systems in bacteria have been used as tools for genome …

Monoterpene indole alkaloids (MIAs) are a class of natural products comprised of thousands
of structurally unique bioactive compounds with significant therapeutic values. Due to …