In this chapter, we provide an overview of the fundamental concepts of DNA transposition
mechanisms. Our aim is to emphasize basic themes and, in this effort, we will focus on …

The development of high-throughput sequencing (next-generation sequencing technology
(NGS)) and the continuous increase in experimental throughput require the upstream …

Viruses have long been considered to be the most promising tools for human gene therapy.
However, the initial enthusiasm for the use of viruses has been tarnished in the light of …

Bacteria and archaea acquire resistance to viruses and plasmids by integrating fragments of
foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific …

Transposons are discrete segments of DNA that have the distinctive ability to move and
replicate within genomes across the tree of life.'Cut and paste'DNA transposition involves …

Tn 10 and Tn5 are composite bacterial transposons (Fig. 1). Both these transposons, as well
as their respective IS elements (IS10 and IS50), transpose by a nonreplicative cut‐and …

The distribution and abundance of transposable elements across the tree of life have
significantly shaped the evolution of cellular organisms, but the underlying mechanisms …

Evolvability can be defined as the capacity of an individual to evolve and thus to capture
adaptive mutations. Transposable elements (TE) are an important source of mutations in …

Gene therapy applications require efficient tools for the stable delivery of genetic information
into eukaryotic genomes. Most current gene delivery strategies are based on viral vectors …

Abstract Mobile Genetic Elements (MGEs) are mosaics of functional gene modules of
diverse evolutionary origin and are generally divergent from the hosts´ genetic background …