A diffusion barrier at the transition zone enables the compartmentalization of signaling
molecules by cilia. The BBSome and the small guanosine triphosphatase Arl6, which …

In eukaryotic cells, intracellular components are organized by the microtubule motors
cytoplasmic dynein-1 (dynein) and kinesins, which are linked to cargos via adaptor proteins …

Regulated transgene expression is an integral component of gene therapies, cell therapies
and biomanufacturing. However, transcription factor-based regulation, upon which most …

The next generation of mammalian cell factories for biopharmaceutical production will be
genetically engineered to possess both generic and product-specific manufacturing …

We engineered short ORFs and used them to control the expression level of recombinant
proteins. These short ORFs, encoding a two-amino acid peptide, were placed upstream of …

Inducible transcription systems play a crucial role in a wide array of synthetic biology circuits.
However, the majority of inducible promoters are constructed from a limited set of tried-and …

Objective To identify the causal gene in a multi-incident US kindred with Parkinson's disease
(PD). Methods We characterized a family with a classical PD phenotype in which 7 …

We describe for the first time the creation of a library of 140 synthetic promoters specifically
designed to regulate the expression of recombinant genes in CHO cells. Initially, 10 …

Predictably regulating protein expression levels to improve recombinant protein production
has become an important tool, but is still rarely applied to engineer mammalian cells. We …

Expression of recombinant genes in HEK293 cells is frequently utilized for production of
recombinant proteins and viral vectors. These systems frequently employ the …