Co-culture of the early human embryo: factors affecting human blastocyst formation in vitro.

YJ Menezo, D Sakkas, L Janny - Microscopy research and …, 1995 - europepmc.org
Co-culture systems have been designed to overcome the embryonic developmental arrest
observed in vitro in conventional culture media. Oviduct and uterine epithelial cells can …

Glucose and glycine synergistically enhance the in vitro development of porcine blastocysts in a chemically defined medium

T Mito, K Yoshioka, S Yamashita… - Reproduction …, 2012 - CSIRO Publishing
In the present study, the effects of glucose and/or glycine on the in vitro development of Day
5 (Day 0= IVF) porcine blastocysts were determined. The addition of 2.5–10 mM glucose to …

Metabolism of pyruvate by pre-elongation sheep embryos and effect of pyruvate and lactate concentrations during culture in vitro

JG Thompson, AC Bell, PA Pugh… - … fertility and development, 1993 - CSIRO Publishing
In the first of two experiments, utilization of [1-14C] pyruvate by 8-cell and blastocyst-stage
embryos derived in vivo was examined during a 3-h incubation in HEPES-buffered synthetic …

Exposure of embryonic cells to alcohol: contrasting effects during preimplantation and postimplantation development

DR Armant, DE Saunders - Seminars in perinatology, 1996 - Elsevier
Alcohol is a known teratogen that causes a broad variety of developmental anomalies,
including fetal growth retardation, craniofacial anomalies, and neurological disorders. The …

A simplified method for the reconstruction of fully competent mouse zygotes from adult somatic donor nuclei

Q Zhou, L Boulanger, JP Renard - Cloning, 2000 - liebertpub.com
Until now, full-term development of mouse embryos reconstructed from somatic nuclei has
been convincingly achieved only when a piezoimpact pipette drive unit is used for the …

One‐minute exposure of 4‐cell mouse embryos to glucose overcomes morula block in CZB medium

CL Chatot, J Lewis‐Williams, I Torres… - Molecular …, 1994 - Wiley Online Library
One‐cell mouse embryos that block at the 2‐cell stage can progress to the morula stage in
CZB medium, but fail to cavitate and then swell and lyse. A 1‐min exposure to 27 mM …

Partial purification of embryotrophic factors from human oviductal cells.

LP Liu, ST Chan, PC Ho… - Human reproduction …, 1998 - academic.oup.com
Embryotrophic factors from human oviductal cells were partially purified by liquid
chromatographic methods. The conditioned medium from human oviductal cell culture was …

Generation of neural progenitors from induced Bama miniature pig pluripotent cells

X Li, ZY Shan, YS Wu, XH Shen, CJ Liu… - …, 2014 - rep.bioscientifica.com
Regarding bull fertility, establishing an association between in vitro findings and field fertility
requires a multi-parametric approach that measures the integrity of various structures and …

Differential expression of glucose transporter isoforms during embryonic stem cell differentiation

S Tonack, A Rolletschek, AM Wobus, B Fischer… - Differentiation, 2006 - Elsevier
In mouse blastocysts six facilitative glucose transporter isoforms (GLUT) 1–4, 8 and 9 are
expressed. We have used the mouse embryonic stem (ES) cell line D3 and spontaneously …

Alleviation of the two-cell block of ICR mouse embryos by polyaminocarboxylate metal chelators.

T Matsukawa, S Ikeda, H Imai… - Reproduction (Cambridge …, 2002 - europepmc.org
The present study was undertaken to examine the effects of various transition metal ion
chelators, both polyaminocarboxylates (including nitrilotriacetate (NTA) …