Programmable gene-editing tools have transformed the life sciences and have shown
potential for the treatment of genetic disease. Among the CRISPR–Cas technologies that …

The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Prime editing (PE) applications are limited by low editing efficiency. Here we show that
designing prime binding sites with a melting temperature of 30° C leads to optimal …

Background It is a long-standing goal of scientists and breeders to precisely control a gene
for studying its function as well as improving crop yield, quality, and tolerance to various …

The recently reported prime editor (PE) can produce all types of base substitution, insertion
and deletion, greatly expanding the scope of genome editing. However, improving the …

Prime editors (PEs), which can install desired base edits without donor DNA or double-
strand breaks, have been used in plants and can, in principle, accelerate crop improvement …

Prime editing is a novel and universal CRISPR/Cas-derived precision genome-editing
technology that has been recently developed. However, low efficiency of prime editing has …